重庆医学2017，Vol.46Issue(6)：735-737,741,4.DOI:10.3969/j.issn.1671-8348.2017.06.005
伊洛前列素对低氧性肺动脉高压大鼠肺部内皮素-1表达的影响
Effects of iloprost on expression of Endothelin-1 in lung in rats with hypoxic pulmonary hypertension
摘要
Abstract
Objective To investigate the influence of iloprost on the expression of lung endothelin-1(ET-1) in rats with hypoxic pulmonary hypertension(HPH).Methods Thirty-two male SD rats were randomly divided into four groups:normal control (NC) group,normal treatment(NT) group,hypoxia control(HC) group,hypoxia treatment (HT) group.NC group and NT group raised under normal Oxygen conditions 3 weeks.HC and HT group placed in a low Oxygen chamber (O2 10％) were treated 3 weeks of hypoxia 8 hours per day.NT and HT group were treated daily iloprost by inhalation therapy (2μg/kg).Affer three weeks,measured mean pulmonary arterial pressure (mPAP),right ventricular systolic pressure (RVSP),index of right ventricular hypertrophy.HE staining was used to observe the morphological changes of pulmonary arteries and image analysis system was used to calculate the percentage of vascular wall thickness of small pulmonary arteries in each group,RT-PCR technique was used to assess the trends of ET-1 in lung tissue homogenates.Results The hemodynamics in HC group was significantly higher than other groups(P＜0.05),there was no significant statistically difference of the hemodynamics in HT group compare with normal control group.Pulmonary arteries morphology,vessel wall thickening and vessel lumina stenosis in HC group than NC、NT、group.These indicators were significant improved in HT group.ET-1 expression in lung tissue were significantly increased in HC group than NC groups.No significant difference was found between and NC group HT group.Conclusion Aerosol inhalation of iloprost has exact therapeutic effect for rats with HPH.Iloprost reduced ET-1 overexpression in lung tissue in HPH rats and prevent pulmonary vascular remodeling.关键词
高血压,肺性/缺氧/伊洛前列素/内皮素-1Key words
hypoxic hypertension, pulmonary/anoxia/iloprost/endothelin-1分类
医药卫生引用本文复制引用
官莉,金柱,陈世雄,向光明,高宝安..伊洛前列素对低氧性肺动脉高压大鼠肺部内皮素-1表达的影响[J].重庆医学,2017,46(6):735-737,741,4.基金项目
湖北省自然科学基金资助项目(2012FFB06301) （2012FFB06301）
湖北省自然科学基金指导性计划项目(2014CFC1037). （2014CFC1037）
