重庆医学2017，Vol.46Issue(6)：738-741,4.DOI:10.3969/j.issn.1671-8348.2017.06.006
和颜坤泰胶囊对卵巢早衰大鼠性激素水平和VEGF、bFGF mRNA表达的影响
Effect of Yan Kuntai capsule on rat sex hormone level and VEGF bFGF mRNA expression in different stages and premature ovarian failure
摘要
Abstract
Objective To explore the Chinese medicine treatment of QUNTAI capsule in different stages of premature ovarian failure effect.Methods Sixty SD female rats were divided into 6 groups:normal group,model group,prevention group,intervention group,the treatment group,and Western medicine group.To observe the changes of behavior in rats,enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of follicle stimulating hormone FSH,LH,serum estradiol E2,and anti Mueller hormone AMH.RT-PCR was used to detect ovarian tissue vascular endothelial growth factor (VEGF) and basic effect of fibroblast growth factor (bFGF) mRNA expression.Results The levels of FSH and LH in the normal group and the administration group were significantly lower compare to model group (P＜0.05),and the contents of AMH and E2 were significantly higher compare to model group (P＜0.05).The FSH and LH content in the model group was significantly higher than that of the normal group and each treatment group,E2,AMH content was significantly lower than that of the normal group and each treatment group (P＜0.05);the expression of VEGF andbFGF mRNA in each treatment group and nornial group was significantly higher than that of model group (P＜0.05),Prevention group was higher than intervention group and treat ment group (P＜0.05),there was no Gignificont difference between prevention group and Western medicine group(P＞0.05).Conclusion QUNTAI capsule can increase serum E2 and AMH levels,lower serum FSH,LH levels,improve ovarian sensitivity to sex hormone levels,repair damaged ovarian tissue,promote follicular growth and development,Thereby inhibiting premature follicle depletion.关键词
坤泰胶囊/卵巢功能早衰/雌激素类/血管内皮生长因子A/碱性成纤维细胞生长因子2Key words
medicine Kuntai/ovarian failure, premature/estrogens/vascular endothelial growth factor A/fibroblast growth factor 2分类
医药卫生引用本文复制引用
徐文君,高慧,李杨,梁策..和颜坤泰胶囊对卵巢早衰大鼠性激素水平和VEGF、bFGF mRNA表达的影响[J].重庆医学,2017,46(6):738-741,4.基金项目
河北省中医药管理局中医药类科研计划课题(2016068) （2016068）
国家中医药管理局全国名老中医药专家高慧传承工作室建设项目(2015048157) （2015048157）
河北省省级重大医学科研项目(zyzd2013018) （zyzd2013018）
河北省政府资助临床医学优秀人才培养项目(2014571024). （2014571024）
