重庆医学2017，Vol.46Issue(7)：868-870,874,4.DOI:10.3969/j.issn.1671-8348.2017.07.002
移植免疫耐受中调节性B细胞与调节性T细胞的依赖性关系研究
Dependence relationship between regulatory B cells and regulatory T cells in transplantation immune tolerance
摘要
Abstract
Objective To investigate the relationship between regulatory B cells (Bregs) and regulatory T cells(Tregs) in transplantation tolerance induced by dual anti-CD45RB/anti-TIM-1 antibody.Methods The longterm murine islet allograft transplant models from BALB/c to C57BL/6 were established.B cells purified from longterm survivors (LTS)were adoptively transferred to grafted B cell-deficient μMT-/-B6 recipients and treated with or without PC61.The allograft survival time was analyzed and the number of Foxp3+ Treg cells was examined by flow cytometry.Murine skin allograft transplant models was established and treated with dual antibody.The change of the percentage of Treg cells was observed after B-cell depletion using anti-CD20 antibody.Results Adoptive transfer of Bregs in longterm tolerated murines could induce longterm tolerance in 83.3 ％ of murines.But after adding CD25m monoclonal antibody,islet grafts were completely rejected in a short time(MST=20 d);adoptive transfer of Bregs in longterm tolerated murines could increase the Tregs number(P＜0.01),moreover could induce CD4+ Foxp-T cell to express Foxp3;after deleting B cells with CD20 antobody,Tregs cells number in the skin transplant recipient murine induced by dual antibody was remarkably reduced (P＜0.01).Conclusion In transplantation tolerance induced by dual antibody,Breg cells may prolong the graft survival time possibly through promoting Treg generation.关键词
移植耐受/调节性B细胞/T淋巴细胞,调节性Key words
transplantation tolerance/regulatory B cells/T-lymphocytes, regulatory分类
医药卫生引用本文复制引用
李姝蓉,赵高平,杨卯竹,魏玲玲,邓绍平..移植免疫耐受中调节性B细胞与调节性T细胞的依赖性关系研究[J].重庆医学,2017,46(7):868-870,874,4.基金项目
国家自然科学基金资助项目(81172832,81571565) （81172832,81571565）
四川省杰出青年基金资助项目(2013JQ0020) （2013JQ0020）
四川省青年科技创新团队专项计划(2014TD0010) （2014TD0010）
四川省卫生和计划生育委员会课题(110190). （110190）
