重庆医学2017，Vol.46Issue(7)：879-882,4.DOI:10.3969/j.issn.1671-8348.2017.07.005
染料木素对尿毒症大鼠细胞外基质沉积的影响
Effect of genistein on deposition of extracellular matrix in uremic rats
王浩 1冯冰 1朱超 1于建平 2黄宝砖3
作者信息
- 1. 解放军第四一一医院肾内科,上海200081
- 2. 第二军医大学长海医院肾内科,上海200433
- 3. 上海市徐汇区中心医院肾内科 200031
- 折叠
摘要
Abstract
Objective To investigate the effect of genistein on deposition of extracellular matrix(ECM) in uremic rats and its mechanism.Methods The uremic rat model was established by 5/6 nephrectomy.The model rats were divided into the genistein group(G) and control group (C).The sham-operation group served as the normal control(S).Urinary protein and biochemical indexes before operation,at postoperative 4,8 weeks were measured in various groups.The pathologic changes of renal tissue were observed.The glomerular sclerosis index(GSI) was evaluated by the semi-quantitative method.fibronectin(FN) and type Ⅳ collagen (ColⅣ) deposition situation were detected by using the immunohistochemical method.Western blot and RT-PCR were used to measure the protein expression and mRNA transcription of TGF-β1.Results Comparing postoperative 4 weeks with the group C,the urinary protein excretion amount was decreased[(11.63 ± 2.07) mg/d vs.(19.93 ± 3.19) mg/d,all P＜ 0.01],serum urea nitrogen and creatinine levels of the group G were decreased[(9.39±0.59)mmol/L vs.(12.09±0.78)mmol/L,(65.11±3.79)mmol/L vs.(77.63±3.20)μmol/L,all P＜0.01].Until postoperative 8 weeks,the urinary protein excretion amount was decreased and the decrease of serum urea nitrogen and creatinine levels was more obvious.The deposition of ColⅣ and FN in renal glomerulus was lower than that of the control group[(17.30±1.96)％ vs.(24.68±3.97)％;(18.26±2.31)％ vs.(29.35±4.15)％,all P＜ 0.01].Glomerular sclerosis was significantly alleviated.The TGF-β1mRNA and protein expression were attenuated (P＜0.05).Conclusion Genistein can reduce the deposition of ECM in uremic rats and has a protective effect on kidney.关键词
染料木素/尿毒症/大鼠,Sprague-Dawley/胶原Ⅳ型/纤维连接蛋白/转化生长因子β1Key words
genistein/uremia/rats, Sprague-Dawley/collagen type Ⅳ/fibronectin/transforming growth factor beta1分类
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王浩,冯冰,朱超,于建平,黄宝砖..染料木素对尿毒症大鼠细胞外基质沉积的影响[J].重庆医学,2017,46(7):879-882,4.
