动物医学进展2017，Vol.38Issue(1)：6-10,5.
小反刍兽疫病毒N蛋白原核表达与间接ELISA检测方法的建立
Prokaryotic Expression of N Protein of Peste des Petits Ruminants Virus and Development of Indirect ELISA
摘要
Abstract
Peste des petits ruminants virus (PPRV) N protein is the major structural protein of virus particles,and it is the main target protein in the diagnosis of PPRV.To establish the expression and purification methods for efficient soluble PPRV N protein and indirect ELISA method for diagnosis of PPRV based on N,this study got a high expression of soluble N protein in Escherichia coli expression system through optimization of codon and expression conditions,and established an indirect ELISA detection method based on N protein.The results showed that the assay was specific and repeatable.Furthermore,a total of 480 clinical serum samples were detected by this assay and the agreement was 98.33% with commercial ELISA Kit (IDVET).This study laid the foundation for the further development of kit for detection of PPRV antibody.关键词
小反刍兽疫/N活性蛋白/可溶性表达与纯化/间接酶联免疫吸附试验Key words
Peste des petits ruminants/N active protein/soluble expression and purification/indirect ELISA分类
农业科技引用本文复制引用
孙雨,赵柏林,王晓英,董浩,翟新验,曲萍,胡冬梅,杨天意,石慧..小反刍兽疫病毒N蛋白原核表达与间接ELISA检测方法的建立[J].动物医学进展,2017,38(1):6-10,5.基金项目
国家重点计划研发项目(2016YFD0500901) （2016YFD0500901）
