动物医学进展2017，Vol.38Issue(2)：12-16,5.
一株猪流行性腹泻病毒S、M、N基因的遗传变异分析
Genetic Correlation and Immunogenicity Analysis of S,M,N Genes ofPorcine Epidemic Diarrhea Virus
易新健 1刘准 1张翠翠 1臧传佼 1徐健 1陈申秒1
作者信息
- 1. 山东滨州沃华生物工程有限公司,山东省动物病原微生物工程实验室,山东滨州 256600
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摘要
Abstract
To carry out the genetic variation and diversity of antigenic sites between CV777 vaccine strain and epidemic strain of porcine epidemic diarrhoea virus,RT-PCR was used to clone the S,M and N genes of PEDV CV777 vaccine strain and JS2016 strain,then the PCR products were sequenced for analyzing the nucleotide sequences and antigenic sites.The nucleotide sequence of CV777 vaccine strain had some variation compared to JS2016 strain,but the homology was higher than 93%.The prediction result of immunogenicity showed that less differences of antigenic sites of S,M and N genes exist between CV777 vaccine strain and JS2016 strain.关键词
猪流行性腹泻病毒/CV777/S基因/M基因/N基因Key words
Porcine epidemic diarrhoea virus/CV777/S gene/M gene/N gene分类
农业科技引用本文复制引用
易新健,刘准,张翠翠,臧传佼,徐健,陈申秒..一株猪流行性腹泻病毒S、M、N基因的遗传变异分析[J].动物医学进展,2017,38(2):12-16,5.
