动物医学进展2017，Vol.38Issue(2)：27-31,5.
鸡心包积液综合征病原的分离鉴定与进化分析
Isolation,Identification and Evolution Analysis of a Fowl Adenovirus Serotype 4 Strain from Chickens with Hydropericardium Syndrome
李昕键 1吴彻 1雷小亚 1严一铭 1戴振凯 1张新珩 1蔺文成 1陈伟国 1陈峰1
作者信息
- 1. 华南农业大学动物科学学院,广东广州 510642
- 折叠
摘要
Abstract
In this research,a fowl adenovirus serotype 4 strain named GD1605 was isolated and identified from livers and hearts of sick chickens in a farm of Hainan province in China,May 2016,based on SPF chicken embryo culture,chicken embryo pathological lesions,PCR assay,BLAST and sequence analysis.The results showed that each of inoculated chicken embryo appeared to be dead 5 d to 7d post inoculation and the death embryoes were observed as arrested development,feather scarce and skin hemorrhage.An avian adenovirus-specific PCR product (884 bp) was amplified and sequenced.The genetic evolution analysis results showed that the virus shared high identity with FAV-4 in group Ⅰ.The genetic evolution analysis also indicated that the virus was closely related to the FAV-4(group I) strains of SDSX,SDSX-15,SDNY2-15,HB1510 and HBLF-15.These results confirmed that the isolated virus is a member of fowl adenovirus serotype 4 in group Ⅰ,and causes hydropericardium-hepatitis syndrome.This virus still endangering chicken flocks in China,the epidemic expands unceasingly,and display the trend of epidemic from north to south regions in China.关键词
心包积液综合征/禽腺病毒/分离/鉴定/序列分析Key words
Hydropericardium-hepatitis syndrome/Fowl adenovirus/isolation/identification/sequence analysis分类
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李昕键,吴彻,雷小亚,严一铭,戴振凯,张新珩,蔺文成,陈伟国,陈峰..鸡心包积液综合征病原的分离鉴定与进化分析[J].动物医学进展,2017,38(2):27-31,5.
