动物医学进展2017，Vol.38Issue(3)：1-4,4.
H5亚型和N6亚型禽流感病毒二重RT-PCR检测方法的建立
Development of a Duplex RT-PCR for Detecting AIV H5 and N6 Subtypes
摘要
Abstract
In order to establish a rapid,simple H5 and N6 subtypes avian influenza virus (AIV) detection method,according to the sequences of HA and NA genes of H5 and N6 subtypes of AIV,two pairs of specific primers were designed respectively,the conditions were optimized and the duplex RT-PCR assay for detection of AIV H5 and N6 subtypes was established.It was shown that H5N6 AIV could be amplified into two specific bands by this duplex RT-PCR, the lengths of bands were 418 bp(H5 AIV) and 251 bp(N6 AIV),respectively.Samples containing H5 or N6 subtype AIV could be amplified into one specific band,which was 418 bp,251 bp,respectively.No specific band was amplified from other subtypes AIV and avian pathogenic virus.Sensitivity test results showed that as low as 1.59×10-5ng/μL H5N6 AIV could be detected.This duplex RT-PCR assay is a specific,sensitive method for the detection of H5 and N6 subtypes AIV in the same tube,and provided an effective tool to determine the infection of AIV H5 and N6 subtypes.关键词
禽流感病毒/H5亚型/N6亚型/二重逆转录-聚合酶链反应Key words
Avian influenza virus/H5 subtype/N6 subtype/duplex RT-PCR分类
农业科技引用本文复制引用
何莹,谢芝勋,罗思思,李孟,谢志勤,谢丽基,黄莉,邓显文,黄娇玲..H5亚型和N6亚型禽流感病毒二重RT-PCR检测方法的建立[J].动物医学进展,2017,38(3):1-4,4.基金项目
广西自然科学基金项目(2015GXNSFBA139070) （2015GXNSFBA139070）
广西科技项目(桂科AD16380009,14123001-8) （桂科AD16380009,14123001-8）
广西水产畜牧局科研项目(桂渔牧科201452001) （桂渔牧科201452001）
南宁市科技项目(20152308) （20152308）
