动物医学进展2017，Vol.38Issue(3)：5-10,6.
2015年-2016年度南宁部分地区猪戊型肝炎病毒基因型分析
Analysis of Swine Hepatitis E Virus Genotype in Nanning City of Guangxi from 2015 to 2016
摘要
Abstract
In oder to investigate genotype of swine hepatitis E virus (HEV) in Guangxi,in this study,104 fresh fecal samples were collected from swine farms around Nanning and screened by nested RT-PCR for HEV virus.The PCR results showed that 11 out of 104 samples were HEV virus positive.The amplified partial ORF2 gene fragments were cloned,sequenced and analyzed.The results showed that nucleotide homology of 11 strains was 97.7%-100% and amino acids homology was 97.9%-100%.It shared a higher nucleotide homology 84.6%-90.6% with HEV genotype 4 Ch-S-1,Ch-T11,swGX40 than that with other HEV genotypes.The phylogenetic tree indicated that 11 HEV strains belonged to HEV genotype 4, most of the HEV strains were clustered in 4a subtype,while a strain classified into subtype 4e.These data presented in this study indicated that the prevalence of HEV strains were genotype 4,subtype 4a was the dominant prevalent subtype in swine in Guangxi.关键词
戊型肝炎病毒/基因型/猪Key words
HEV/genotype/swine分类
农业科技引用本文复制引用
贺微,姚静,覃一峰,林思远,邹柳,黄加滨,韦莹,欧阳康,陈樱..2015年-2016年度南宁部分地区猪戊型肝炎病毒基因型分析[J].动物医学进展,2017,38(3):5-10,6.基金项目
国家自然科学基金项目(31372444) （31372444）
广西大学科研基金项目(XGZ130959) （XGZ130959）
