动物医学进展2017，Vol.38Issue(3)：38-43,6.
宁夏某肉牛场牛支原体分离鉴定及病理组织学观察
Isolation and Identification of Mycoplasma bovis and Its Histopathological Observation in a Beef Cattle Farm of Ningxia
摘要
Abstract
For isolation and identification of Mycoplasma bovis and analysis of target organ damage in a beef cattle farm of Ningxia,this study used Thiaucourt's liquid filter medium and solid medium to isolate pathogens,designed universal 16 S rRNA primers and uvrC specific primers of Mycoplasma bovis to amplifiy and sequence the gene,used DNA Star software to compare the sequence of the isolated strain and the standard strains in GenBank,and MEGA6.0 software Neighbor-joining method to construct and analyze phylogenetic tree of isolates based on 16 S rRNA and uvrC sequences,and used paraffin section technology and hematoxylin-eosin staining for histopathological observation.The results showed that the bacterial colony looks like "fried egg",16 S rRNA and uvrC amplified fragments were consistent with the positive control,phylogenetic trees constructed based on 16 S rRNA and uvrC gene sequences were in the same branch with Mycoplasma bovis;lung tissue structure disappeared,part of alveolar consolidation and collapse,focal pulmonary hemorrhage,alveolar infiltration with inflammatory cells;thickening of alveolar septa,shedding of a small amount of alveolar epithelial cells;alveolar wall fracture,alveolar inflammatory exudate,fibrous connective tissue proliferation.关键词
牛支原体/分离/鉴定/病理组织学Key words
Mycoplasma bovis/isolation/identification/histopathology分类
农业科技引用本文复制引用
郭亚男,曹思婷,何生虎,王爽,张鑫,孙鹏..宁夏某肉牛场牛支原体分离鉴定及病理组织学观察[J].动物医学进展,2017,38(3):38-43,6.基金项目
宁夏科技攻关重点专项项目(312-0164) （312-0164）
