南方医科大学学报2016，Vol.36Issue(12)：1621-1625,5.DOI:10.3969/j.issn.1673-4254.2016.12.06
雌激素通过调节AKT信号通路活性抑制肝癌细胞的侵袭和转移
Oestrogen inhibits invasion and metastasis of hepatocellular carcinoma MHCC97H cells by regulating the activity of AKT signaling pathway
摘要
Abstract
Objective To explore the inhibitory effect of estrogen against metastasis of human hepatocellular carcinoma MHCC97H cells and explore the molecular mechanism.Methods The inhibitory effect of estrogen on the migration and invasion of MHCC97H cells was evaluated with wound healing assay and Transwell assay.Western blotting was used for investigating the expression of MMP-2,MMP-9,AKT and p-AKT in the cells treated with estrogen.Results Estrogen treatment significantly inhibited the migration and invasion of MHCC97H cells in a dose-dependent manner.Estrogen significantly down-regulated the protein expressions of MMP-2 and MMP-9 and lowered the phosphorylation level of AKT.Conclusion The anti-metastatic effect of estrogen involves inhibition of MMP-2 and MMP-9 in MHCC97H cells probably by regulating AKT signal pathway.关键词
雌激素/MHCC97H细胞/侵袭/AKT信号通路/基质金属蛋白酶Key words
estrogen/MHCC97H cells/invasion/AKT signal pathway/metalloproteinases引用本文复制引用
田长印,张欣,赵文学,党双锁,靳耀锋,纪泛扑..雌激素通过调节AKT信号通路活性抑制肝癌细胞的侵袭和转移[J].南方医科大学学报,2016,36(12):1621-1625,5.基金项目
国家自然科学基金(81300322) （81300322）
陕西省科技公关项目(2008K09-05) （2008K09-05）
西安交通大学第二附属医院人才培养专项基金(RC(GG)201501)Supported by National Natural Science Foundation of China (81300322). （RC(GG）
