南方医科大学学报2016，Vol.36Issue(12)：1638-1649,12.DOI:10.3969/j.issn.1673-4254.2016.12.09
PPAR-γ2基因-C34G、NADPH氧化酶p22phox亚基基因-C242T多态性与幽门螺杆菌感染的交互作用和食管鳞状细胞癌的关系
Interaction of polymorphisms of PPAR-γ2 gene-C34G and NADPH oxidase subunit p22phox gene-C242T with Helicobacter pylori infection in esophageal squamous cell carcinoma
摘要
Abstract
Objective To investigate the interaction of polymorphisms of PPAR-γ2 gene-C34G and NADPH oxidase subunit p22phox gene-C242T with helicobacter pylori (H.pylori) infection in esophageal squamous cell carcinoma (ESCC).Methods A total of 200 cases of LSCC of Broder grade Ⅰ,200 of Broder grade Ⅱ and of grade Ⅲ were enrolled in this study with 200 healthy individuals as the control group.The genetic polymorphisms of PPAR-γ2 gene-C34G and NADPH oxidase subunit p22phox gene-C242T were analyzed using PCR-RFLP in peripheral blood leukocytes.14C-urea breath test (14C-UBT) was used to test 14C disntegration per minute (DPM) for evaluating the infection status of H.pylori.An unconditional logistic regression model was used to analyze the interaction of nucleotide polymorphisms and H.pylori infection.Results The risk of ESCC significantly increased in subjects with-C34G (CG),-C34G(GG),-C242T (CT),and-C242T (TT) genotypes.Combined analysis of the polymorphisms showed that the subjects carrying-C34G (GG)/-C242T (TT) had a high risk of ESCC,and a positive interaction was found between-C34G (GG) and-C242T (TT) in increasing the risk of ESCC.Positive interactions in the pathogenesis of ESCC were also found between-C34G (CG) and-C242T (TT),between-C34G (CG) and-C242T (CT),and between-C34G (GG) and-C242T (CT) (γ＞1).The risk of ESCC significantly increased in subjects with H.pyloriinfection,which showed positive interactions with-C34G (CG),-C34G (GG),-C242T (CT) and-C242T (TT) in increasing the risk of ESCC (γ＞1).Conclusion Individuals carrying-C34G(CG),-C34G(GG),-C242T (CT) and-C242T (TT) genotypes have a high risk of developing ESCC,and these genotypes interact with H.pylori infection in the pathogenesis of LSCC,suggesting the importance of eradicating H.pylorifor prevention of ESCC.关键词
食管鳞状细胞癌/过氧化物酶体增殖因子活化受体-γ2基因-C34G/烟酰胺腺嘌呤二核苷酸磷酸氧化酶p22phox亚基基因-C242T/幽门螺杆菌/多态现象Key words
esophageal squamous cell carcinoma/Helicobacter pylori infection/PPAR-γ2 gene-C34G/NADPH oxidase subunit p22phox gene-C242T/gene polymorphisms引用本文复制引用
张超贤,郭李柯,张利利,韩宇..PPAR-γ2基因-C34G、NADPH氧化酶p22phox亚基基因-C242T多态性与幽门螺杆菌感染的交互作用和食管鳞状细胞癌的关系[J].南方医科大学学报,2016,36(12):1638-1649,12.基金项目
河南省教育厅科研基金(20073200143) （20073200143）
