南方医科大学学报2016，Vol.36Issue(12)：1667-1671,5.DOI:10.3969/j.issn.1673-4254.2016.12.13
Mir-106b表达与食管鳞状细胞癌的关系
Expression of mir-106b in esophageal squamous cell carcinoma
摘要
Abstract
Objective To investigate the expression of mir-106b in esophageal squamous cell carcinoma (ESCC) tissue and analyze its correlation with the cilnicopathologic features of ESCC.Methods A total of 200 fresh surgical specimens of ESCC and adjacent tissues collected between 2001 and 2007 were examined for expressions of mir-106b using real-time PCR (RT-PCR).Northern blot analysis for mir-106b was performed in 4 pairs of samples to confirm the RT-PCR results.The relationship between mir-106b expression and clinicopathological features and prognosis of the patients were analyzed.Results Mir-106b was expressed at significantly higher levels in ESCC tissues than in the paired adjacent tissues.Overexpression of mir-106b was associated with lymph node metastasis,stage of TNM classification and smoking (P＜0.05).The survival rate of patients with low mir-106b expression was higher than that of patients with high mir-106b expression (60 vs 37 months,P=0.024).Cox regression analysis indicated that the expression of mir-106b,lymph node metastasis and smoking were independent prognostic factors for ESCC (P＜0.05).Conclusion Mir-106b is overexpressed in ESCC tumors,and its overexpression is strongly associated with lymph node metastasis and a poor prognosis.Mir-106b expression is an independent prognostic factor for ESCC and can serve as a biomarker for diagnosis and prognostic evaluation of ESCC.关键词
mir-106b/过表达/预后/食管鳞状细胞癌Key words
mir-106b/overexpression/prognosis/esophageal squamous cell carcinoma引用本文复制引用
肖长艳,郭子健,曹磊,丁晓文,刘强,王小春..Mir-106b表达与食管鳞状细胞癌的关系[J].南方医科大学学报,2016,36(12):1667-1671,5.基金项目
国家自然科学基金(81272511) （81272511）
天津市自然科学基金重点项目(14JCZDJC35800) （14JCZDJC35800）
北京协和医学院放射医学研究所青年探索基金(2016ZX310072) （2016ZX310072）
北京市化工职业防治院院长基金(BHY1601001)Supported by National Natural Science Foundation of China (81272511). （BHY1601001）
