南方医科大学学报2017，Vol.37Issue(2)：141-149,9.DOI:10.3969/j.issn.1673-4254.2017.02.01
中国南方汉族人群20个常染色体STR基因座的多态性分析
Polymorphism analysis of 20 autosomal short-tandem repeat loci in southern Chinese Han population
摘要
Abstract
Objective To evaluate the value of PowerPlex（R）21 System (Promega) and study the genetic polymorphism of its 20 short-tandem repeat (STR) loci in southern Chinese Han population.Methods We conducted genotyping experiments using PowerPlex(R)21 System on 20 autosomal STR loci (D3S1358,D1S1656,D6S1043,D13S317,Penta E,D16S539,D18S51,D2S1338,CSF1PO,Penta D,TH01,vWA,D21S11,D7S820,D5S818,TPOX,D8S1179,D12S391,D19S433 and FGA) in 2367 unre1ated Chinese Han individuals living in South China.The allele frequencies and parameters commonly used in forensic science were statistically analyzed in these individuals and compared with the reported data of other populations.Results The PowerPlex（R） 21 System had a power of discrimination (PD) ranging from 0.7839 to 0.9852 and a power of exclusion (PE) ranging from 0.2974 to 0.8099 for the 20 loci.No significant deviation from Hardy-Weinberg expectations was found for all the loci except for D5S818.This southern Chinese Han population had significant differences in the allele frequencies from 8 ethnic groups reported in China,and showed significant differences at 8 to 20 STR foci from 5 foreign populations.The allele frequency at the locus D1S1656 in this southern Chinese Han population differed significantly from those in the 5 foreign populations and from 3 reported Han populations in Beijing,Zhejiang Province and Fujian Province of China.The neighbor-joining phylogenetictree showed clustering of all the Asian populations in one branch,while the northern Italian and Argentina populations clustered in a separate branch.This southern Chinese Han population had the nearest affinity with the Yi ethnic population in Yunnan Province of China.Conclusion The 20 STR loci are highly polymorphic in this southern Chinese Han population,suggesting the value of this set of STR loci in forensic personal identification,paternity testing and anthropological study.关键词
短串联重复序列/群体遗传学/中国南方汉族Key words
short-tandem repeat/population genetics/Han population引用本文复制引用
陈玲,陆慧洁,杜蔚安,邱平明,刘超..中国南方汉族人群20个常染色体STR基因座的多态性分析[J].南方医科大学学报,2017,37(2):141-149,9.基金项目
广东省自然科学基金(2014A030310025) （2014A030310025）
广东省医学科研基金(A2015043) （A2015043）
法医遗传学公安部重点实验室开放课题(2015FGKFKT03)Supported by Natural Science Foundation of Guangdong Province (2014A030310025),Open Project of Key Laboratory of Forensic Genetics in Ministry of Public Security (2015FGKFKT03) and Medical Science and Technology Research Foundation of Guangdong Province (A2015043). （2015FGKFKT03）
