南方医科大学学报2017，Vol.37Issue(2)：172-177,6.DOI:10.3969/j.issn.1673-4254.2017.02.05
全反式视黄酸预防性给药可减轻类风湿关节炎模型大鼠的炎症
Prophylactic administration of all-trans retinoic acid alleviates inflammation in rats with collagen-induced arthritis
摘要
Abstract
Objective To investigate the effects of prophylactic administration of all-trans retinoic acid (ATRA) in relieving inflammation in a rat model of collagen-induced arthritis (CIA).Methods Female Wistar rats (6 to 8 weeks old) were randomly divided into normal control group,solvent control group,and prophylactic ATRA treatment (0.05,0.5,and 5 mg/kg) groups.All the rats except for those in normal control group were subjected to subcutaneous injection of type Ⅱ collagen and incomplete Freund adjuvant in the tails to induce CIA,followed by injection on the following day with saline,corn off or different doses of ATRA 3 times a week.The arthritis index (AI) scores,histological scores,serum levels of TNF-α,IL-17A,and IL-10,and expressions of proteases related with cartilage damage were evaluated.Results On the 15th day after the primary immumza tion,the AI scores increased significantly in all but the normal control groups;the scores increased progressively in all the 3 ATRA groups but remained lower than that in the solvent control group,which was stable over time.The rats in the 3 ATRA groups showed obvious pathologies in the knee and ankle joints,but the semi-quantitative scores of pathology damage showed no significance among them.Compared with those in solvent control group,the serum IL-17A and TNF-α levels decreased,serum IL-10 level increased,and the expressions of ADAMT-4 and MMP-3 proteins decreased significantly in the knees in the 3 ATRA groups.Conclusion ATRA can reduce the production of TNF-α and IL-17A and increase the production of IL-10 to alleviate the inflammation in rats with CIA.ATRA may delay the progression of RA by correcting the imbalance of Th1/Th2 and Th17/Treg.关键词
类风湿关节炎/胶原诱导性关节炎/全反式视黄酸Key words
rheumatoid arthritis/collagen-induced arthritis/all-trans retinoic acid引用本文复制引用
曾香,单靖焱,刘杨,宁艳花,谢雪健,申雨燕,宋杰,李云..全反式视黄酸预防性给药可减轻类风湿关节炎模型大鼠的炎症[J].南方医科大学学报,2017,37(2):172-177,6.基金项目
国家自然科学基金(81372983)Supported by National Natural Science Foundation of China (81372983). （81372983）
