南方医科大学学报2017，Vol.37Issue(2)：178-183,6.DOI:10.3969/j.issn.1673-4254.2017.02.06
红景天苷通过抑制NOX2-ROS-MAPKs信号途径保护H2O2诱导的PC12细胞凋亡
Salidroside protects PC12 cells from H2O2-induced apoptosis via suppressing NOX2-ROS-MAPKs signaling pathway
摘要
Abstract
Objective To investigate the molecular mechanism by which salidroside protects PC12 cells from H2O2-induced apoptosis.Methods PC12 cells cultured in DMEM supplemented with 10％ horse serum and 5％ fetal bovine serum were pretreated with different doses of salidroside for 2 h and then stimulated with H2O2 for different lengths of time.The expression levels of PARP and caspase 3 and the phosphorylation of p38,ERK and JNK were determined with Western blotting.The cell nuclear morphology was observed after DAPI staining.The production of ROS was detected using a ROS detection kit,and the levels of gp91phox and p47phox in the membrane and cytoplasm were detected by membrane-cytoplasm separation experiment;the binding between gp91phox and p47phox was assayed by coimmunoprecipitation experiment.Results Salidroside dose-dependently suppressed cell apoptosis,lowered phosphorylation levels of p38,ERK and JNK,inhibited the production of ROS,reduced the binding between gp91phox and p47phox,and inhibited the activity of NOX2 in PC12 cells exposed to H2O2.Conclusion Salidroside protects PC12 cells from H2O2-induced apoptosis at least partly by suppressing NOX2-ROS-MAPKs signaling pathway.关键词
红景天苷/神经保护/NOX2/ROS/MAPKsKey words
salidroside/neuroprotection/NOX2/reactive oxygen species/mitogen-activated protein kinases引用本文复制引用
戚之琳,刘银华,齐世美,凌烈锋,冯遵永,李强..红景天苷通过抑制NOX2-ROS-MAPKs信号途径保护H2O2诱导的PC12细胞凋亡[J].南方医科大学学报,2017,37(2):178-183,6.基金项目
国家自然科学基金(81601380) （81601380）
安徽高校自然科学研究项目重大项目(KJ2016SD59) （KJ2016SD59）
安徽省优秀青年人才支持计划重点项目(gxyqZD2016173) （gxyqZD2016173）
皖南医学院校重点科研项目培育基金(WK2015201) （WK2015201）
皖南医学院博士启动基金(WK2014RC05)Supported by National Natural Science Foundation of China (81601380). （WK2014RC05）
