南方医科大学学报2017，Vol.37Issue(2)：192-198,7.DOI:10.3969/j.issn.1673-4254.2017.02.08
复发性外阴阴道假丝酵母菌病患者阴道微生物的动态变化
Longitudinal analysis of vaginal microbiota in women with recurrent vulvovaginal candidiasis
摘要
Abstract
Objective To investigate the vaginal flora in patients with recurrent vulvovaginal candidiasis (RVVC).Methods Vaginal swabs were collected at different time points from 6 RVVC patients and 5 healthy women of child-bearing age.The dynamic changes,microbiota composition,alpha diversity and beta diversity in the two groups were assessed by analyzing the 16S rRNA V4 hypervariable region amplified from the total genomic DNA from the swabs.Results Lactobacillus was the predominant species in healthy women with similar proportions of Liners and L.crispatus;small proportions of Gardnerella,Prevotella and other genus were also detected.In some healthy women,the vaginal flora showed a high relative abundance of anaerobic bacteria such as Gardnerella,Prevotella,Atopobium,Sneathia.Compared with the healthy women,patients with RVVC showed a significantly reduced diversity of vaginal flora,where Liners was the predominant species and the content of L.crispatus decreased significantly.In healthy women,the vaginal flora fluctuated with the menstrual cycle,and the fluctuation was the most prominent during menstruation;the dominant species either alternated regularly or maintain an absolute superiority in the menstrual cycle.The vaginal flora showed attenuated fluctuation in women with RVVC,were highly conserved within the menstrual cycle,and maintained a similar composition in the episodes and intermittent periods.Conclusion The vaginal flora of RVVC patients do not undergo regular variations with the menstrual cycle and shows a similar composition between the episodes and intermittent periods.Promoting the production of L.iners or inhibiting the colonization of L.crispatus to restore the composition of the vaginal flora may help in the treatment of RVVC.关键词
复发性外阴阴道假丝酵母菌病/阴道菌群/动态分析/BIPES/16S rRNAKey words
recurrent vulvovaginal candidiasis/vaginal flora/dynamic analysis/BIPES/16S rRNA引用本文复制引用
马啸,蔡慧华,何彦,郑慧敏,康玲,周宏伟,刘木彪..复发性外阴阴道假丝酵母菌病患者阴道微生物的动态变化[J].南方医科大学学报,2017,37(2):192-198,7.基金项目
国家自然科学基金(31570497)Supported by National Natural Science Foundation of China (31570497). （31570497）
