南方医科大学学报2017，Vol.37Issue(2)：226-231,6.DOI:10.3969/j.issn.1673-4254.2017.02.14
桃儿七可促进慢性粒细胞白血病K562细胞的凋亡
Effects of Sinopodophyllum hexundrum on apoptosis in K562 cells
摘要
Abstract
Objective To investigate the effects of Sinopodophyllum hexundrum on apoptosis in K562 cells.Methods K562 cells were treated with Sinopodophyllum hexundrum at different concentrations and for different lengths of time to determine the optimal conditions of SinoPodophyllum hexandrum treatment for K562 cells using CCK8 assay.The cell apoptotic rate was detected by flow cytometry,and the cell morphology and nuclear morphology of K562 cells were observed with Wright staining and DPAI staining,respectively.The protein expressions of BCR/ABL,p-BCR/ABL,STAT5,p-STAT5 and the apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were determined with Western blotting.Results The ceil proliferation was inhibited in a concentration-and time-dependent manner by 1,2,and 3 μg/mL Sinopodophyllum hexundrum.The treatment was optimal with a Sinopodophyllum hexundrum concentration of 2 μg/mL a treatment time of 48 h,and the cell apoptotic rate increased in a time-dependent manner and significantly increased at 48 h (P＜0.001).The expression of apoptosis-related proteins PARP,caspase-3 and cleaved-caspase-3 were also activated in a time-dependent manner.The cells showed typical apoptotic changes after treatment with 2 μg/mL Sinopodophyllum hexundrum for 48 h with significantly reduced expressions of BCR/ABL,p-BCR/ABL,STAT5,AND p-STATS.Conclusion Sinopodophyllum hexundrum promotes K562 cell apoptosis possibly by inhibiting BCR/ABL-STAT5 survival signal pathways and activating the mitochondrion-associated apoptotic pathways.关键词
桃儿七/慢性粒细胞白血病/BCR/ABL/STAT5信号通路/凋亡Key words
Sinopodophyllum hexundrum/chronic myeloid leukemia/BCR/ABL/STATS signal pathways/cell apoptosis引用本文复制引用
周芳竹,王欣,代安亚,黄峥兰,李会,黄宁姝,冯文莉..桃儿七可促进慢性粒细胞白血病K562细胞的凋亡[J].南方医科大学学报,2017,37(2):226-231,6.基金项目
重庆市卫生和计生委中医药科技项目(ZY201402109) （ZY201402109）
