南方医科大学学报2017，Vol.37Issue(2)：238-244,7.DOI:10.3969/j.issn.1673-4254.2017.02.16
宣威地区肺腺癌病人肺组织特异miRNAs表达谱和靶基因及其信号通路预测
Specific microRNA expression profiles of lung adenocarcinoma in Xuanwei region and bioinformatic analysis for predicting their target genes and related signaling pathways
摘要
Abstract
Objective To identify differentially expressed microRNAs (miRNAs) related to lung adenocarcinoma in Xuanwei region and predict their target genes and related signaling pathways based on bioinformatic analysis.Methods High-throughput microarray assay was performed to detect miRNA expression profiles in 34 paired human lung adenocarcinoma and adjacent normal tissues (including 24 cases in Xuanwei region and 10 in other regions).Gene ontology and KEGG pathway analyses were used to predict the target genes and the regulatory signaling pathways.Results Thirty-four miRNAs were differentially expressed in lung adenocarcinoma tissues in cases in Xuanwei region as compared with cases in other regions,including 23 upregulated and 11 downregulated miRNAs.The predicted target genes included GF,RTK,SOS,IRS1,BCAP,CYTOKINSR,ECM,ITGB,FAK and GβY involving the PI3K/Alt,WNT and MAPK pathways.Conclusion The specific microRNA expression profiles of lung adenocarcinoma in cases found in Xuanwei region allow for a better understanding of the pathogenesis of lung adenocarcinoma in Xuanwei.The predicted target genes may involve the PI3K/Alt,WNT and MAPK pathways.关键词
miRNA芯片/miRNAs/宣威肺腺癌/靶基因/信号通路Key words
micrRNAs chip/miRNAs/lung adenocarcinoma/target genes/signaling pathways引用本文复制引用
陈帅,周永春,陈颖,陈小波,李光剑,杨加鹏,雷玉洁,赵光强,黄秋博..宣威地区肺腺癌病人肺组织特异miRNAs表达谱和靶基因及其信号通路预测[J].南方医科大学学报,2017,37(2):238-244,7.基金项目
国家自然科学基金(U1202224,81460441) （U1202224,81460441）
云南省应用基础研究面上项目(2016FB145) （2016FB145）
云南省卫生科技计划项目(2014NS001) （2014NS001）
云南省医疗卫生单位内设研究基金(2016NS106) （2016NS106）
云南省肿瘤医院博士科研启动基金(BSJJ201502)Supported by National Natural Science Foundation of China (U1202224,81460441). （BSJJ201502）
