中国耳鼻咽喉头颈外科2017，Vol.24Issue(3)：144-148,5.DOI:10.16066/j.1672-7002.2017.03.009
颗粒物PM10和CD4+T淋巴细胞IL-4 DNA甲基化在儿童变应性鼻炎发病机制中的研究
Pathogenesis of particulate matters and IL-4 DNA methylation in CD4+T cells in children with AR
摘要
Abstract
OBJECTIVE To investigate the possible effects of meteorological and environmental factors on IL-4 gene specific DNA methylation levels in CD4+T cells of children with AR.METHODS Thirty five pediatric AR patients(6-12 years) followed up for one year from the Department of Otolaryngology, Shanghai Children Medical Center from Jan, 1, 2012 to Dec. 31, 2012 were included in this study. Data on daily particulate matter of diameter smaller than 10 micrometer(PM10) and particulate matter of diameter smaller than 2.5 micrometer(PM2.5) was available as average values derived from the data of 6 state-controlled monitoring stations distributed across Pudong District, Shanghai. We quantified IL-4(interleukin-4) gene specific DNA methylation levels in CD4+T cells from 35 patients with AR and 30 healthy controls. mRNA levels of IL-4 gene were measured by real-time reverse transcriptase-PCR. Methods of personal exposure assessment of PM2.5 and PM10 were measured.RESULTS Compared with controls, IL-4 promoter region was hypomethylated in AR CD4+T cells(P=0.038). Of all observed CpG sites in IL-4 promoter region, there was significant differences in CpG-48, CpG+54(P=0.041, 0.032). IL-4 mRNA expression was significantly increased in CD4+T cells(P=0.039). The level of IL-4 mRNA expression was negatively correlated to the mean level of methylation in IL-4 promoter region. After adjusting, level of PM10 exposure was negatively correlated with level of methylation in IL-4 promoter region(r2=0.419,β=-0.470,SD=0.781,P=0.045). CONCLUSION Level of methylation in IL-4 promoter region may be affected by PM10 exposure.关键词
儿童/鼻炎,变应性,常年性/空气污染物/白细胞介素4/表观基因组Key words
Child/Rhinitis/Allergic/Perennial/Air Pollution/Interleukin-4/epigenomics引用本文复制引用
李幼瑾,李牛,牟喆,马蓓颖,陈洁,江帆..颗粒物PM10和CD4+T淋巴细胞IL-4 DNA甲基化在儿童变应性鼻炎发病机制中的研究[J].中国耳鼻咽喉头颈外科,2017,24(3):144-148,5.基金项目
国家自然科学基金资助项目(81502815)、上海市科研技术委员会科研计划项目(15ZR1427000)、上海市科研技术委员会引导类项目(15401933800)和上海交通大学医工交叉研究基金项目(YG2016MS29)联合资助 （81502815）
