儿科药学杂志2017，Vol.23Issue(3)：1-5,5.DOI:10.13407/j.cnki.jpp.1672-108X.2017.03.001
雾化给药对重组人干扰素-α2b生物活性和分子结构的影响
The Effects of Atomization Inhalation on Biological Activity and Molecular Structure of Human Recombinant Interferon Alpha 2b
王兵 1程婷 2卢晨 1周乐春 1倪晓燕 1李增礼 1王荣海 1宋礼华1
作者信息
- 1. 安徽安科生物工程(集团)股份有限公司,安徽合肥230088
- 2. 安徽省生物研究所,安徽合肥230032
- 折叠
摘要
Abstract
Objective:To evaluate the effects of atomization inhalation of administration with recombinant human interferon alpha 2b (rhIFN-αt2b) on its biological activity and molecular structure.Methods:The rhIFN-α2b biological activity was detected by virus inhibition method.The influence of the air jet on IFN-α2b biological activity was studied by comparing its biological activity before and after atomization.The changes of IFN-oα2b molecular structure were analyzed by high performance liquid chromatography (HPLC) method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method.Results:Atomization inhalation of administration didn' t cause the peptide bond of IFN-α2b protein to break,but air jet could cause disulfide bond to break.The ratio of IFN-αt2b molecular with disulfide bond breaking was related to its concentration before atomization.After atomization the purity of 6 millions IU/mL,3 millions IU/mL and 1 million IU/mL IFN-α2b were 82.9％,90.1％ and 94.9％ respectively.For 6 millions IU/mL concentration rhIFN-α2b solution,after atomization would cause disulfide bond of 17.1％ rhIFN-α2b molecular to break.Therein a pair of two disulfide bonds breaking occured in 0.5％ of rhIFN-α2b molecules.And the retention rate of rhIFN-α2b biological activity after atomization in remainder fluid was about 98.2％ and in condensed fluid was 96.1％.Conclusion:Recombinant human interferon α2b can be transmitted stably in spray pattern.Its biological activity can be retained basic completely.The molecular structure of most of IFN-α2b keeps perfectly after the atomization.Single or double disulfide bond breaking in a few molecules of rhIFN-α2b is caused by air jet.In order to minimize the loss of rhIFN-α2b molecules,it is suggested atomization rhIFN-α2b concentration should be controlled under 1 million IU/mL in clinical treatment.关键词
重组人干扰素-α2b/雾化给药/生物活性/分子结构/二硫键Key words
recombinant human interferon alpha 2b/atomization inhalation of administration/biological activity/molecular structure/disulfide bond分类
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王兵,程婷,卢晨,周乐春,倪晓燕,李增礼,王荣海,宋礼华..雾化给药对重组人干扰素-α2b生物活性和分子结构的影响[J].儿科药学杂志,2017,23(3):1-5,5.
