发光学报2017，Vol.38Issue(3)：288-295,8.DOI:10.3788/fgxb20173803.0288
基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测
InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA
摘要
Abstract
The fluorescent nanoprobe for the detection of DNA was established by utilizing mercaptopropionic acid coated InP@ZnS QDs and Dured.In this nanoprobe.The InP@ZnS QDs/Dured fluorescent nanoprobes were structured by electrostatic interactions between environment friendly,negatively charged InP@ZnS quantum dots and positively charged Dured,and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer (FRET).At presence of DNA,DNA and Dured were specific binding,which enable Dured removing from the surface of InP@ZnS QDs and achieving fluorescence recovery of InP@ZnS QDs.Thus,the detection of DNA was realized.The linear range of the InP@ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0-275.0 ng·L-1 and 1.0 ng·L-1,respectively.The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions.关键词
InP@ZnS量子点/荧光探针/DNA/荧光共振能量转移Key words
InP@ZnS quantum dots/fluorescent probe/DNA/FRET分类
数理科学引用本文复制引用
胡先运,孟铁宏,张汝国,江家志,黄星宏..基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J].发光学报,2017,38(3):288-295,8.基金项目
国家自然科学基金(21545006) （21545006）
贵州省自然科学基金(2015GZ48861,2016GZ13752) （2015GZ48861,2016GZ13752）
中央高校基本科研业务费专项资金(KYLX0187) （KYLX0187）
黔南民族医专科研基金(QNYZ201601)资助项目 Supported by National Natural Science Foundation of China(21545006) （QNYZ201601）
Natural Science Foundation of Guizhou Province(2015GZ48861,2016GZ13752) （2015GZ48861,2016GZ13752）
Special Fund for Basic Scientific Research of Central University(KYLX0187) （KYLX0187）
National Medical Research Foundation of Qiannan(QNYZ201601) （QNYZ201601）
