福建农业学报2016，Vol.31Issue(12)：1257-1261,5.DOI:10.19303/j.issn.1008-0384.2016.12.001
鸭瘟病毒的分离鉴定及其UL 2、TK 基因序列分析
Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL 2 and TK Genes
摘要
Abstract
In March 201 5,massive deaths happened to the M18 Duck Farm and an egg-laying duck farm in Fuzhou. It was suspected of an outbreak of duck plaque.Samples of liver and esophagus from the dead birds were collected for pathogenic identification using PCR and virus isolation.Two strains with virulent characteristics were thus identified.Subsequently,a sequence analysis on their UL 2 genes further confirmed the classification.In addition, the TK gene sequences of the two isolated virus strains showed a nucleotide homology of 98.7% with those of the common virulent strain as well as the vaccine strain.关键词
鸭瘟病毒/分离鉴定/UL 2 基因/TK 基因/序列分析Key words
virulent duck plague virus/isolation and identification/UL 2 gene/TK gene/sequence analysis分类
农业科技引用本文复制引用
刘荣昌,陈红梅,黄瑜,卢荣辉,傅秋玲,江斌,万春和,傅光华,程龙飞,施少华..鸭瘟病毒的分离鉴定及其UL 2、TK 基因序列分析[J].福建农业学报,2016,31(12):1257-1261,5.基金项目
国家水禽产业技术体系项目(CARS-43) （CARS-43）
福建省畜禽疫病防控技术重大研发平台(2014N2003) （2014N2003）
福建省科技计划项目---省属公益类科研院所基本科研专项(2017R1102) （2017R1102）
福建省农业科学院科研项目(2015 QC-2) （2015 QC-2）
“国家百千万工程人才”科研补助项目(NCNCMTPC-2009) （NCNCMTPC-2009）
