福建农业学报2016，Vol.31Issue(12)：1267-1272,6.DOI:10.19303/j.issn.1008-0384.2016.12.003
16SrRNA和COI基因条形码在12种观赏鱼种类鉴定中的应用
1 6S rRNA and COI Gene Barcodes Applied for Identification of Ornamental Fishes
摘要
Abstract
Partial sequences of 16S rRNA genes and mitochondrial cytochrome oxidase subunit I (COI)genes from 1 2 highly valuable ornamental fish species were amplified using universal primers.The species included the red-tail golden arowana, the yellow-tail arowana, the green arowana,Scleropages formosus,Scleropages jardinii, Osteoglossumbicirrhosum,Pterophyllumscalare,Hemichromisletourneuxi,Aulonocarastuartgranti,Helostoma temminkii,Poeciliareticulate and Trichopodus leerii.The homology and phylogenetic analyses on the genes were carried out by using DNA sequence analysis software,and compared with the databases at GenBank and Bold Systems.The sequence analysis indicated that the information on 16S rRNA and COI genes might be applicable for distinguishing S.formosus,S.jardinii,O.bicirrhosum and P.reticulate from one another.However,the phylogenetic tree constructed with the COI gene would be more closely related to the conventional classification than 16S rRNA.Regardless,the use of 16S rRNA and/or COI gene was not deemed sufficient to differentiate or identify very similar genera or species,such as cichlids or different lines of a same species,nor among the red-tail arowana, the yellow-tail arowana and the green arowana.It was suggested that DNA barcoding with higher evolution rate be studied to overcome the shortcoming of the current method.关键词
基因条码/观赏鱼/物种鉴定/16SrRNA/COIKey words
gene barcode/ornamental fish/species identification/16S rRNA/COI分类
生物科学引用本文复制引用
陈信忠,郭书林,龚艳清,袁芳,许奕宸,杨俊萍..16SrRNA和COI基因条形码在12种观赏鱼种类鉴定中的应用[J].福建农业学报,2016,31(12):1267-1272,6.基金项目
福建省海洋经济发展专项(2015N0015) （2015N0015）
