福建农业学报2016，Vol.31Issue(12)：1273-1279,7.DOI:10.19303/j.issn.1008-0384.2016.12.004
木薯蔗糖磷酸合成酶基因克隆及组织表达分析
Cloning and Tissue Expressions of Sucrose Phosphate Synthase Gene in Cassava
摘要
Abstract
Sucrose phosphate synthase (SPS)gene from cassava RS01 was cloned to study its biological functions. The conserved fragment of the gene was first obtained by homologous cloning,and its full length cDNA by rapid amplification of cDNA ends (RACE).The characteristics,gene sequence and other biological information retrieved were analyzed.Expressions of the genes from various tissues of the plants at different times were compared by RT-qPCR.The results showed that the cDNA of SPS gene had a full length of 3,857 bp,and an ORF of 3,228 bp encoding a protein of 1,076 amino acids.It was subsequently named MeSPS with a GenBank number of KX822780. Its homologous alignments with the nucleotide and protein databases were highly homologs with those of J atropha carcas L.,Ricinus communis L.,and Litchi chinensis Sonn.at 89%,89%,and 87%,respectively.Its amino acid sequence was 77% - 92% in similarity with the SPS genes of other plants.The qRT-PCR analysis showed that MeSPS gene was highly expressed during seeding,and,was more expressed in the leaves than the stems or roots on a plant in a same growth period.关键词
木薯/蔗糖磷酸合成酶/基因克隆/表达分析Key words
cassava/sucrose phosphate synthase/gene cloning/expression analysis分类
农业科技引用本文复制引用
黄堂伟,罗兴录,单忠英,朱艳梅..木薯蔗糖磷酸合成酶基因克隆及组织表达分析[J].福建农业学报,2016,31(12):1273-1279,7.基金项目
国家“973”计划项目(2010CB126601) （2010CB126601）
广西科学研究与技术开发计划项目(桂科重14121005-2-1) （桂科重14121005-2-1）
南宁市科学研究与开发计划项目(201109044B) （201109044B）
国家现代农业产业体系广西薯类产业创新团队项目(nycytxgxcxtd-03-11-2) （nycytxgxcxtd-03-11-2）
南宁市科学研究与技术开发计划项目(20132307) （20132307）
