福建农业学报2016，Vol.31Issue(12)：1345-1352,8.DOI:10.19303/j.issn.1008-0384.2016.12.016
利用物质组学方法筛选产游离油酸的芽胞杆菌菌株
Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics
摘要
Abstract
Extracellular metabolites in the supernatants 52 Bacillus-like strains were analyzed by HPLC along with the quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS ). Subsequently, the molecular feature extraction (MFE)was performed on the data using the software MassHunter followed by submitting the information for comparison against the mass spectrometry database,Metlin.Free oleic acid was thereby detected in the cultural supernatant of Bacillus plakortidis ,FJAT-14858. Its matching score with oleic acid was 95.61,accurate mass was 284.2693,and relative content in the metabolites collected was up to 1.62%.To date,B .plakortidis FJAT-14858 was the first Bacillus-like bacterium known to produce free oleic acid with no cytotoxicity to the bacteria per se.关键词
海绵芽胞杆菌 FJAT-14858/游离油酸/物质组学/高效液相色谱-四级杆飞行时间质谱Key words
Bacillus plakortidis FJAT-14858/free oleic acid/chemomics/HPLC-quadrupole time-of-flight MS分类
生物科学引用本文复制引用
肖通奋,王阶平,刘波,陈峥,刘国红,陈倩倩,车建美,葛慈斌..利用物质组学方法筛选产游离油酸的芽胞杆菌菌株[J].福建农业学报,2016,31(12):1345-1352,8.基金项目
国家自然科学基金(31370059) （31370059）
福建省重大专项项目农业科技重大专项(2015NZ0003-1) （2015NZ0003-1）
福建省种业创新与产业化工程项目(FJZZZY-1544) （FJZZZY-1544）
福建省农业科学院人才引进科研启动基金项目(YJRC2014-1) （YJRC2014-1）
福建省科技计划项目---省属公益类科研院所基本科研专项(2014R1101016-11) （2014R1101016-11）
