口腔疾病防治2017，Vol.25Issue(3)：143-152,10.DOI:10.12016/j.issn.2096-1456.2017.03.002
Notch信号通路在颞下颌骨关节炎中的表达
Expression of Notch signaling pathway in temporomandibular osteoarthritis
摘要
Abstract
Objective To investigate the expression of Notch signaling molecules in temporomandibular joint ar-thritis (TMJOA), and to explore the role and mechanism of Notch signaling pathway in the development and progression of TMJOA. Methods 72 Kunming mice were randomly divided into experimental group, sham-surgery group and nor-mal group. In experimental group, discs on the right TMJ were subjected to total discectomy, sham-operation group un-derwent the same procedure without disc removal, while normal group serve as blank control. All the left temporoman-dibular joint discs were not treated. 8 mice in each group were sacrificed respectively at 1 week, 2 week, 4 week after surgery. Histological examinations were performed to assess success of TMJOA model, according to the pathological standard of osteoarthritis diagnosis. Immunochemistry techniques were performed in the successful TMJOA cartilages to evaluate the expression levels of Notch1 (NICD1), Jagged1, Hes1 and Hes5. The scores were evaluated by semi-quantita-tive method. Results Notch1 (NICD1), Jagged1 and Hes5 were activated in the experimental group with the expression levels increased dramatically over time. While Hes1 expression was suppressed at the beginning of osteoarthritis but was up-regulated afterwards. Conclusion Notch pathway-related molecular expression changed greatly in TMJOA mod-el, indicating that the pathway in the occurrence and development of TMJOA plays an important role.关键词
Notch信号通路/颞下颌关节/骨关节炎/关节盘摘除/软骨Key words
Notch signaling pathway/Temporomandibular joint/Osteoarthritis/Discectomy/Cartilage分类
医药卫生引用本文复制引用
兰玲莉,张伟娜,蒋扬眉,李婷,应彬彬,祝颂松..Notch信号通路在颞下颌骨关节炎中的表达[J].口腔疾病防治,2017,25(3):143-152,10.基金项目
国家自然科学基金(81070850) （81070850）
国家自然科学基金(81271170) （81271170）
浙江省自然科学基金(LY12H14002) （LY12H14002）
