口腔疾病防治2017，Vol.25Issue(3)：153-158,6.DOI:10.12016/j.issn.2096-1456.2017.03.003
TLR4在人健康和深龋牙髓组织中的定位表达研究
Locations of TLR4 in healthy dental pulp tissue and dental pulp tissue affected by deep caries
摘要
Abstract
Objective To analyze the role of TLR4 in innate immune response of dental pulp by comparing the lo-cations and expressions of TLR4 in healthy dental pulp tissue and dental pulp tissue affected by deep caries. Meth-ods Healthy teeth and teeth affected by deep caries were demineralized and stained with hematoxylin and eosin (HE) to observe the morphology of dental pulp. Immunohistochemistry staining was performed to observe the expressions of TLR4. Results Observed under HE staining, dentin tubules of teeth affected by deep caries were damaged with a lot bacteria mass. The expression of TLR4 were located in the odontoblast layer and near the blood vessels in both groups. Positive staining of TLR4 in deep caries pulps (2.10±0.74) were significantly higher than that in healthy teeth (1.25 ± 0.46). Conclusion Expression of TLR4 in deep caries pulp is stronger than that in healthy pulp. It suggests that TLR4 may play a role in the innate immune response of deep caries.关键词
牙髓/TLR4/深龋/定位表达/天然免疫反应Key words
Dental pulp tissue/TLR4/Deep caries/Location/Innate immune response分类
医药卫生引用本文复制引用
刘影,高岩,文军,徐帅妹..TLR4在人健康和深龋牙髓组织中的定位表达研究[J].口腔疾病防治,2017,25(3):153-158,6.基金项目
广东省自然科学基金项目(2015A030310071) （2015A030310071）
广东省医学科学技术研究基金项目(A2016356) （A2016356）
广东省医学科学技术研究基金项目(A2016212) （A2016212）
广东省自筹经费类科技计划项目(粤科规财字[2015]110号) （粤科规财字[2015]110号）
