广东医学2017，Vol.38Issue(1)：65-71,7.
SIRT1对肺癌A549细胞增殖和迁移的影响
SIRT1 promotes the migration of lung cancer cells A549 through influencing EMT
摘要
Abstract
Objective To investigate the influence of SIRT1 on cell proliferation and migration in A549 cells. Methods lung cancer A549 cells were culture .The recombinant adenovirus was applied to up -regulate/down-regu-late the expression of SIRT1.MTT assay, colony formation, wound-healing assay and trans -well migration assay were performed to measure multiplication capacity and migration ability .Western Blot assay was performed to detect the influ-ence of down -regulating SIRT1 on the expression of EMT related proteins , including ZO -1, β-catenin, Snail and ZEB1.Results After up -regulating/down -regulating the expression of SIRT1, the proliferation rate of A549 cell showed no obvious change , but healing rate and migration ability of A 549 cells were enhanced/weakened .The level of EMT related epithelial markers ZO -1 was increased, while the level of interstitial markers β-catenin, Snail and ZEB1 were decreased after down -regulating the expression of SIRT1.Conclusion SIRT1 promotes lung cancer cell A549 mi-gration through influencing EMT related proteins .关键词
SIRT1/肺癌/增殖/迁移/EMTKey words
SIRT1/lung cancer/proliferation/migration/EMT引用本文复制引用
于娜,陈建明,李晓才,张海涛,汪亚君,伍俊,张佳喜..SIRT1对肺癌A549细胞增殖和迁移的影响[J].广东医学,2017,38(1):65-71,7.基金项目
国家自然科学基金青年基金项目(编号:81400023) ,广东医科大学优秀硕士论文培育项目(编号:4CX14091G) （编号:81400023）
