广东医学2017，Vol.38Issue(4)：501-504,4.
青蒿琥酯联合来那度胺对多发性骨髓瘤耐药细胞株的作用
The study on combination effect of artesunate and lenalidomide on the treatment of bortezomib-resistant multi-ple myeloma cell line
摘要
Abstract
Objective To investigate the combination effect of artesunate and lenalidomide on the proliferation of bortezomib-resistant multiple myeloma cell line MM .1R, and its molecular mechanism .Methods MM.1R cells were cultured in RPMI 1640 and treated with DMSO (control group), lenalidomide (20 μmol/L), artesunate (25 μg/mL), or lenalidomide (20μmol/L) plus artesunate (25μg/mL), separately.After 24 or 48 h, the cell proliferation rates were assessed by MTT assay, and the NF-κB (P65), Bcl-xl, Mcl-1, p-Bad, and P-gp protein levels were measured by Western blot .Results The inhibitory rate of cell proliferation was 15.16%by lenalidomide for 24 h, 28.28%by lena-lidomide for 48 h, 45.98%by artesunate for 24 h, 17.19%by artesunate for 24 h, 34.53%by lenalidomide plus artesu-nate for 24 h, or 65.16%by lenalidomide plus artesunate for 48 h (P<0.05).NF-κB (P65), Bcl-xl, Mcl-1, p-Bad, and P-gp proteins were down -regulated by lenalidomide plus artesunate treatment in a time -dependent manner . Conclusion Both artesunate and lenalidomide inhibit MM .1R cell proliferation .Combination of artesunate with lenalido-mide can enlarge this effect through down -regulating NF-κB (P65), Bcl-xl, Mcl-1, p-Bad, and P-gp.关键词
青蒿琥酯/来那度胺/骨髓瘤/耐药Key words
artesunate/lenalidomide/multiple myeloma/drug-assistant引用本文复制引用
欧瑞明,周长华,谭友平,刘爽,钟启,张青,杜苑苑,郑丽玲,刘志..青蒿琥酯联合来那度胺对多发性骨髓瘤耐药细胞株的作用[J].广东医学,2017,38(4):501-504,4.基金项目
国家自然科学基金资助项目(编号: 81400168 ) ,广东省医学科研基金立项项目(编号: A2013128 ) ,广东省中医药局建设中医药强省科研课题(编号:20131104 ) ,广东省第二人民医院引进人才科研启动基金(编号: YY2014-002) （编号: 81400168 ）
