广东医学2017，Vol.38Issue(4)：509-513,5.
Salubrinal对大鼠脑缺血再灌注模型LC3-ⅡmRNA及LC3-Ⅱ/LC3-ⅠmRNA的影响
Effects of salubrinal on LC3-ⅡmRNA and LC3-Ⅱ/LC3-ⅠmRNA in rat model of cerebral ischemia reper-fusion
摘要
Abstract
Objective To investigate the effects of salubrinal on the expression of LC 3-Ⅱ mRNA and LC3-Ⅱ/LC3-ⅠmRNA in rat model of cerebral ischemia reperfusion .Methods MCAO ischemia reperfusion model in rats were established by using thread bolt method .The rats were randomly divided into sham operation group , model group and salubrinal group (Sal group).Each group was subdivided into 4 subgroups:6 h, 12 h, 24 h, and 72 h subgroups.In each group, the neurological severity score was performed at the corresponding time points after reperfusion , and the rats were sacrificed for brain samples .HE staining and real time -PCR were performed .Results Compared with the sham operation group , the model group and the Sal group had neurological deficits at all time points ( P<0.01 ) .Compared with the model group , neurological deficits in Sal group were significantly alleviated 24 h and 72 h after reperfusion ( P<0.05).Reduced or even absence of neurons , cells swelling or ruptured, nuclear condensed with deep stained , and glial cell proliferated were observed in model group; which were alleviated after salubrinal intervention .Compared with the sham operation group , LC3-ⅡmRNA and LC3-Ⅱ/LC3-ⅠmRNA of the model group and the Sal group were all in-creased at different time points (P<0.01).Compared with the model group, the LC3-ⅡmRNA expression in Sal group was significantly decreased (P<0.05) 12 h, 24 h and 72 h after reperfusion; with LC3-Ⅱ/LC3-Ⅰ mRNA signifi-cantly reduced at each time point after reperfusion ( P<0.01 ) .Conclusion LC3-Ⅱ mRNA and LC3-Ⅱ/LC3-ⅠmRNA are reduced by salubrinal , which protects rats from injury caused by cerebral ischemia reperfusion .关键词
Salubrinal/缺血再灌注大鼠模型/LC3-ⅡmRNA/LC3-Ⅱ/LC3-ⅠmRNA/神经保护Key words
Salubrinal/MCAO brain ischemic reperfusion rats model/LC3-Ⅱ mRNA/LC3-Ⅱ/LC3-ⅠmRNA/nerve protection引用本文复制引用
刘利娟,陈瑶,周德生,王洪海,周颖璨,李中,胡华..Salubrinal对大鼠脑缺血再灌注模型LC3-ⅡmRNA及LC3-Ⅱ/LC3-ⅠmRNA的影响[J].广东医学,2017,38(4):509-513,5.基金项目
湖南省教育厅科学研究重点项目(编号:14 A111 ) ,长沙市科技计划项目(编号:K1308023-31) （编号:14 A111 ）
