国际医药卫生导报2017，Vol.23Issue(6)：771-785,15.DOI:10.3760/cma.j.issn.1007-1245.2017.06.001
神经妥乐平对H2O2致PC12细胞损伤的保护作用
Neuroprotective effect of Neurotropin on H2O2-induced injury in pheochromocytoma cells
摘要
Abstract
Objective To investigate the effect of Neurotropin on H2O2-induced oxidative stress injury in pheochromocytoma (PC12) cells and its potential mechanism.Methods The CCK-8 assay was applied to measure the cell viability while the flow cytometry was used to detect the occurrence of cells oxidative injury,the level of intracellular reactive oxygen species (ROS) and changes of mitochondrial membrane potential.The ROS was observed by fluorescence microscope,and the mRNA expressions of Caspase-3,Bax,and Bcl-2 were detected by immunofluorescence and quantitative real time polymerase chain reaction (qRT-PCR).Results As the increase of H2O2 concentration,the survival rate of PC 12 cells was decreased gradually.The treatment with 450 μM of H2O2 for 24 h induced the cell viability,apoptosis and necrosis significantly and increased the level of the ROS evidently;the red/green fluorescence ratios of JC-1 were decreased in the mitochondrial membrane potential;the mRNA expressions of Bax and Caspase-3 were significantly increased,while the mRNA expression of Bcl-2 was decreased (P＜0.05,compared with the control group).In contrary,with pretreatment of Neurotropin (0.01 UN/ml) for 12 h,it obviously enhanced the cell viability and mRNA expressions of Bcl-2,decreased the rates of apoptosis and necrosis,lessened the accumulation of the ROS,increased the mitochondrial membrane potential,and inhibited the expressions of Bax and Caspase-3 (P＜0.05,compared with the H2O2 group).Conclusion Neurotropin can inhibit the H2O2-induced injury in PC12 cells.Its neuroprotective role may relate to decreasing the level of the ROS,maintaining the high energy of the mitochondrial membrane potential,inhibiting the expression of proapoptotic gene expression and promoting anti-apoptotic gene expression.关键词
神经妥乐平/PC12细胞/氧化应激损伤/线粒体膜电位/凋亡Key words
Neurotropin/PC12 cells/Oxidative stress injury/Mitochondrial membrane potential/Apoptosis引用本文复制引用
方文丽,范胜诺,廖旺,郑雨秋,廖劭伟,刘军..神经妥乐平对H2O2致PC12细胞损伤的保护作用[J].国际医药卫生导报,2017,23(6):771-785,15.基金项目
National Natural Science Foundation of China in 2013 (81372919) （81372919）
Natural Science Foundation of Guangdong Province (2014A030313016)2013年国家自然科学基金(81372919) （2014A030313016）
广东省自然科学基金项目(2014A030313016) （2014A030313016）
