广东药学院学报2017，Vol.33Issue(1)：88-93,6.DOI:10.16809/j.cnki.2096-3653.2016092101
精眯对脾虚大鼠肠黏膜损伤的修复及葡萄糖吸收的影响
Effect of spermidine on the repair of intestinal mucosa injury1 and glucose absorption in rats with spleen deficiency
摘要
Abstract
Objective To observe the effect of spermidine on the repair of intestinal mucosa injury and glucose absorption in rats with spleen deficiency. Methods The successful model SD rats induced by reserpine were randomly divided into five groups, including the model group, three different dosages of spermidine groups and the positive medicine EGF group. Meantime, the healthy SD rats were used as the normal group. Single-track intestinal perfusionin vivo was applied to detect the glucose uptake. Pathological section was used to observe the repair of intestinal mucosa. The changes of odium-dependent glucose transporter ( SGLT-1 ) and easy oxidation of glucose transporter ( GLUT-2 ) were detected by immunohistochemistry and RT-PCR. Results According to the absorption rate constant Ka and effective permeability Papp , the absorption of glucose in the model group was decreased in comparison with the normal group. However, the absorption of glucose in spermidine groups was increased in a dose-dependent mannerwhen compared with the model group. Treatment with spermidine obviously improved intestinal mucosa damage and the expression of SGLT-1 and GLUT-2 mRNA and protein in model rats, especially in high dosage of spermidine group. Conclusion Spermidine promotes the absorption of glucose by upregulation of the intestinal mucosa repair.关键词
精眯/脾虚大鼠/葡萄糖吸收/SGLT-1/GLUT-2Key words
spermidine/spleen deficiency rats/glucose uptake/SGLT-1/GLUT-2分类
医药卫生引用本文复制引用
吴燕燕,黄丽花,王晶,王素军,臧林泉,王桂香..精眯对脾虚大鼠肠黏膜损伤的修复及葡萄糖吸收的影响[J].广东药学院学报,2017,33(1):88-93,6.基金项目
国家自然科学基金项目(81302895) （81302895）
