广东药学院学报2017，Vol.33Issue(1)：112-116,5.DOI:10.16809/j.cnki.2096-3653.2016091203
miR-375逆转MCF-7/Adr耐药性的功能研究
Effect of miR-375 on the resistance of MCF-7/Adr cells
摘要
Abstract
Objective To study the drug resistance of breast carcinoma MCF-7/Adr cells after transfection of miR-375. Methods The miR-375 mimics ( miR-375 mimics ) and mimic negative control ( NC ) were transfected into MCF-7/Adr cells. The proliferation of MCF-7/Adr cells was detected by WST-1 after adriamycin treatment. Cell cycle and apoptosis were detected by flow cytometry. Cell scratch test, transwell migration and invasion assay were used to detect MCF-7/Adr cell migration and invasion. Results Transfection of miR-375 increased the sensitivity of MCF-7/Adr cells to adriamycin and promoted cell apoptosis. Moreover, high level of miR-375 induced cell cycle arrest in G1 phase. Cell migration and invasion ability were also inhibited in MCF-7/Adr cells after miR-375 transfection. Conclusion High expression of miR-375 in MCF-7/Adr cells can enhance its sensitivity to adriamycin.关键词
miR-375/乳腺癌/耐药性Key words
miR-375/breast cancer/drug resistance分类
医药卫生引用本文复制引用
张卉宁,尤琪,黎昆东,张娜,陈娟,张巨峰..miR-375逆转MCF-7/Adr耐药性的功能研究[J].广东药学院学报,2017,33(1):112-116,5.基金项目
国家自然科学基金项目(81171447) （81171447）
广东省科技计划项目(2014A020212303) （2014A020212303）
广东大学生科技创新培育专项资金项目(pdjh2016b0262) （pdjh2016b0262）
