国际神经病学神经外科学杂志2017，Vol.44Issue(1)：35-38,4.DOI:10.16636/j.cnki.jinn.2017.01.008
p38丝裂原活化蛋白激酶在实验性蛛网膜下腔出血后早期脑损伤中的作用
Role of p38 mitogen-activated protein kinase in early brain injury after experimental subarachnoid hemorrhage
摘要
Abstract
Objective To investigate the role of p38 mitogen-activated protein kinase (p38 MAPK) in early brain injury (EBI) after experimental subarachnoid hemorrhage (SAH).Methods A total of 54 adult male Sprague-Dawley (SD) rats were equally and randomly divided into SAH group,control group,and p38 MAPK intervention group.An SAH model was established using intravascular silk puncture.The intervention group was given an intracerebroventricular injection of SB203580 (a specific inhibitor of p38 MAPK) at 30 minutes before modeling.All SD rats were sacrificed at 24 hours after modeling.The brain water content and neurological function score were determined.The expression of p38 MAPK in the brain was measured by RT-PCR and immunohistochemistry.Results The SAH group had significantly higher brain water content and mRNA level of p38 MAPK and a significantly lower neurological function score compared with the control group (t =-196.35,P ＜ 0.01;t =-24.75,P ＜ 0.01;t =201.08,P ＜ 0.01).The p38 MAPK in tervention group had significantly lower brain water content and mRNA level of p38 MAPK and a significantly higher neurological function score compared with the SAH group (t =75.67,P ＜ 0.01;t =9.43,P ＜ 0.01;t =-81.68,P ＜ 0.01).The immunohistochemistry results showed that p38 MAPK protein was expressed only in the SAH group and the p38 MAPK intervention group,and the p38 MAPK group had significantly lower expression of p38 MAPK protein than the SAH group (t =-3.37,P ＜ 0.01).Conclusions p38 MAPK plays an important role in the formation of EBI,and it may be a new drug target for the prevention and treatment of EBI.关键词
蛛网膜下腔出血/早期脑损伤/p38丝裂原活化蛋白激酶/炎症反应Key words
subarachnoid hemorrhage/early brain injury/p38 mitogen-activated protein kinase/inflammatory response/rat引用本文复制引用
孙新刚,马乾,王改青,王荔,胡为民..p38丝裂原活化蛋白激酶在实验性蛛网膜下腔出血后早期脑损伤中的作用[J].国际神经病学神经外科学杂志,2017,44(1):35-38,4.基金项目
山西医科大学第二医院博士基金项目(201501-7) （201501-7）
