光学精密工程2017，Vol.25Issue(2)：319-324,6.DOI:10.3788/OPE.20172402.0319
单分散上转换纳米荧光微粒的荧光寿命测量
Measurement of fluorescence life time of single up-conversion nanoparticle
摘要
Abstract
In order to characterize the photon luminescence properties of up-conversion nanoparticles (UCNPs),a system to measure fluorescence life time of single nanoparticle was established based on a custom-built two-photon microscope scanned with a pair of galvo-mirrors.First,a sample of mono-disperse UCNPs was imaged by the two-photon microscope.Then,the exact location of each nanoparticle was precisely determined through the single molecule fluorescence localization algorithm.The laser was focused on the nanoparticles one by one with a laser pulse with 500μs duration exerted on the nanoparticle.A photomultiplier was employed to measure the time-lapse fluorescence intensity which varied with time.Finally,the fluorescence decay curve was fitted to calculate the life time of single nanoparticle.The experiment results show that the fluorescence curves of single nanoparticle fit with single exponential decay,and the fluorescence life time is 195.3 μs.For contrast,the life time of the bulk sample is 358.9 μs.This indicates a dramatic effect of particle aggregation on the photon luminescence properties of UCNPs.关键词
光学显微成像/上转换纳米荧光微粒/荧光寿命/双光子显微镜Key words
optical microscopy/up-conversion nanoparticles/fluorescence life time/two-photon microscope分类
机械制造引用本文复制引用
李辉,桂征宇,梁永,俞樟森,吴爱国..单分散上转换纳米荧光微粒的荧光寿命测量[J].光学精密工程,2017,25(2):319-324,6.基金项目
国家重大科学仪器设备开发专项资助项目(No.ZDYZ2013-1) （No.ZDYZ2013-1）
国家自然科学基金委面上项目(No.61475185,No.U1432114) （No.61475185,No.U1432114）
