南方农业学报2017，Vol.48Issue(2)：197-201,5.DOI:10.3969/j:issn.2095-1191.2017.02.197
24份古荔枝种质资源ISSR遗传多样性分析
Genetic diversity of 24 ancient litchi germplasm resources using ISSR molecular marker
摘要
Abstract
[Objective]The present study explored the genetic diversity and relationships of Guangxi ancient litchi germplasm resources in order to provide references for utilization of germplasm resources and variety breeding. [Method]The genetic relationships of 24 ancient litchi varieties were analyzed using ISSR molecular marker. [Result]Thirteen primers with clear bands and favorable repeatability were selected from 100 ISSR primers; 96 bands were amplified altogether and 43 of them were polymorphic bands with a polymorphism proportion of 44.79%, and 7.4 bands were amplified by each primer on average. The genetic similarity coefficients among the tested ancient litchi germplasm resources ranged from 0.43 to 1.00 with average relative genetic similarity coefficient of 0.61. UPGMA cluster analysis results indicated that , the 24 tested germplasm resources of ancient litchi could be classified into three groups when genetic similarity coefficient was 0.66. GroupⅠcame from Lingshan county, Guangxi;GroupⅡderived from Guiping city, Beiliu cuty, Yulin city, Lingshan county and Daxin county in Guangxi; Group Ⅲ came from Beiliu city and Lingshan county in Guangxi. [Conclusion]The hereditary basis among the 24 varieties of ancient litchi in Guangxi is extensive , and they can serve as excellent ma-terials for cross breeding of litchi in the future.关键词
古荔枝/ISSR分子标记/遗传多样性/聚类分析Key words
ancient litchi/ISSR molecular marker/genetic diversity/cluster analysis分类
农业科技引用本文复制引用
陆贵锋,黄川,刘钰,廖慧茜,丁峰,罗红卫,李冬波,李鸿莉,彭宏祥..24份古荔枝种质资源ISSR遗传多样性分析[J].南方农业学报,2017,48(2):197-201,5.基金项目
Special Fund for National Litchi and Longan Industrial Technology System(CARS-33-03) （CARS-33-03）
Special Scientific Research Fund for Guangxi Academy of Agricultural Sciences(GXAAS 2013YM16) （GXAAS 2013YM16）
Special Fund for Guangxi Innovation Team Con-struction(nycytxgxcxtd-03-12) （nycytxgxcxtd-03-12）
