南方农业学报2017，Vol.48Issue(2)：297-301,5.DOI:10.3969/j:issn.2095-1191.2017.02.297
脱皮大环柄菇化学成分分析及其抗氧化活性评价
Chemical compositions and antioxidant activity of Macrolepiota detersa
摘要
Abstract
[Objective]The chemical compositions of Macrolepiota detersa were analyzed and its antioxidant activity was evaluated to provide theoretical references for its development and utilization . [Method]Volatile oil and acetone crude extract were obtained from fresh fruit bodies of M. detersa by acetone extraction and vacuum rotatory steam distillation. Chemical compositions of the volatile oil were analyzed by gas chromatography-mass spectrometry (GC-MS) method,and chemical constituents of acetone crude extract were analyzed by pre-test tube method. Antioxidant activity of acetone crude extract was evaluated by free radical scavenging experiment. [Result]GC-MS results showed the main components in the volatile oil were ketone compounds, and the main component 4-hydroxy-4-methyl-2-pentanone accounted for 86.32%. Acetone crude extract contained alkaloids,sterols,and terpenes according to pre-test tube method. Free radical scavenging experiment indicated that acetone crude extract contained antioxidant activity, and half maximal effective concentration (EC50) values of·OH radical scavenging activity was 260.45 μg/mL while EC50 values of·DPPH radical scavenging activity was 552.87 μg/mL. [Conclusion]The main components in volatile oil of M. detersa include seven ketone compounds, one alcohol compound and one ester compound. Acetone crude extract contains alkaloids, sterols, and terpenes, thus embodies antioxidant activity. Therefore M. detersa is worth further development.关键词
脱皮大环柄菇/挥发油/化学成分/抗氧化活性/气相色谱—质谱联用技术Key words
Macrolepiota detersa/volatile oil/chemical composition/antioxidant activity/gas chromatography-mass spectrometry(GC-MS)分类
农业科技引用本文复制引用
朱峰,宋照风,王春燕..脱皮大环柄菇化学成分分析及其抗氧化活性评价[J].南方农业学报,2017,48(2):297-301,5.基金项目
广东省自然科学基金项目(2016A030313380) （2016A030313380）
