南方农业学报2017，Vol.48Issue(2)：350-356,7.DOI:10.3969/j:issn.2095-1191.2017.02.350
广西猪源致病性大肠杆菌对抗菌药物 的耐药性变化分析
Antimicrobial resistance variation of pathogenic Escherichia coli isolated from pig in Guangxi
摘要
Abstract
[Objective]The present study determined antimicrobial resistance of pathogenic Escherichia coli isolated from pig in Guangxi in 2007 and 2016,so as to provide a foundation for the use of antimicrobials in veterinary clinical med-ication for prevention and treatment of porcine colibacillosis. [Method]Two hundred samples of intestinal content, rectum cotton swab, or mesentery lymph node of diarrhea piglets were collected from twenty-five pig farms in Guangxi to isolate E. coli, pathogenicity of the isolates was detected. One hundred and three pathogenic strains were selected for in vitro an-timicrobial sensitivity test. The results were compared with those in 2007. [Result]One hundred and seventy-eight E. coli strains were isolated from the samples with isolation rate of 89%, one hundred and forty-three strains of them were deadly to lab mice. In terms of drug susceptibility of pathogenic E. coli from pig, its drug susceptibility toward 50% of antimi-crobials(nine kinds) increased while the other 50%decreased in 2016 compared to 2007. The increase of drug susceptibi-lity of spectinomycin was the maximum ( 34 . 9%) while the decrease of drug susceptibility of florfenicol was the maxi-mum(46.9%). Intermediary rate of ceftriaxone and doxycycline remained stable, but intermediary rate of other 16 antimi-crobials decreased in 2006 compared to 2007. The most obvious one is cephradine, which reduced by 55.2%. The statistical results of resistance rate showed that only three kinds of antimicrobial ( amikacin , spectinomycin and doxycycline ) de-creased by 12.3%-15.5%,while the other fifteen kinds of antimicrobial increased by 1.2%-62.1%,and resistance rate of amoxicillin increased by 62.1%, resistance rate of florfenicol increased 57.0%. The results showed that, 75.7% of the strains isolated in 2007 were resistant to 5-11 kinds of antimicrobial agents, while 74.8% of the strains isolated in 2016 were resistant to 11 kinds of antimicrobial agents. [Conclusion]At present, pathogenic E. coli isolated from pig in Guangxi are mainly broad spectrum resistant, drug susceptibility and intermediary rate decrease and drug resistance rate increases greatly. Amikacin and spectinomycin can be used in production for prevention and treatment of porcine colibacillosis.关键词
猪源致病性大肠杆菌/抗菌药物/耐药性Key words
porcine pathogenic Escherichia coli/antimicrobial/drug resistance分类
农业科技引用本文复制引用
冯世文,李军,潘艳,彭昊,胡帅,杨威,陈泽祥..广西猪源致病性大肠杆菌对抗菌药物 的耐药性变化分析[J].南方农业学报,2017,48(2):350-356,7.基金项目
广西科学研究与技术开发计划项目(桂科重14121003-4-1) （桂科重14121003-4-1）
广西水产畜牧兽医局科技项目(桂渔牧科201528005) （桂渔牧科201528005）
