广西医科大学学报2017，Vol.34Issue(3)：327-331,5.DOI:10.16190/j.cnki.45-1211/r.2017.03.002
IGF1R3'UTR多态性位点rs3743249与miR-141结合差异与前列腺癌发展风险关系的研究
Study on the relationship between IGF1R 3'UTR polymorphism locus rs3743249 caused variation on miR-141 binding and the developmental risk of prostate cancer
摘要
Abstract
Objective:To study the relationship of IGF1R 3'untranslated region (UTR) polymorphism rs3743249 caused variation of binding affinity to miR-141 and the risk of prostate cancer (PCa).Methods:MiRNAs which could bind to rs3743249 were predicted by miRbase.Expression of IGF1R mRNA and predicted targeted miRNAs were both analyzed from the Cancer Genome Atlas (TCGA) and GEO datasets met inclusion criteria.PCa-associated miRNAs were selected and the binding affinity to alleles of rs3743249 were validated by dual luciferase reporter assay in human embryonic kidney cell (HEK293T) in vitro.Results:miR-141 was predicted to be binding to rs3743249 by analysis of database.IGF1R mRNA was upregulated significantly in PCa tissues in TCGA datasets (P ＜0.05).MiR-141 showed significant upregulation in PCa tissues in TCGA and GEO datasets (GSE23022 and GSE36803) compared with adjacent tissues of paired(P ＜0.05).Dual luciferase reporter assay indicated that miR-141 showed a stronger binding affinity with rs3743249 in the 3'UTR of IGF1R,suggesting that miR-141 played a stabilizing role,rather than an inhibitory role.Conclusion:Polymorphism rs3743249 with G allele inside IGF1R 3'UTR shows a greater risk in the progression of PCa.关键词
前列腺癌/IGF1R/多态性位点/miR-141Key words
prostate cancer/IGF1R/polymorphism/miR-141分类
医药卫生引用本文复制引用
王慧敏,胡艳玲..IGF1R3'UTR多态性位点rs3743249与miR-141结合差异与前列腺癌发展风险关系的研究[J].广西医科大学学报,2017,34(3):327-331,5.基金项目
国家自然科学基金资助项目(No.81272853 （）
No.81472414 （）
No.1370857) （）
