广西医科大学学报2017，Vol.34Issue(3)：337-341,5.DOI:10.16190/j.cnki.45-1211/r.2017.03.004
三维共培养模型下人脐静脉内皮细胞对T24膀胱癌细胞侵袭、迁移及增殖能力的影响
Influence of co-culturing human umbilical vein endothelial cells with T24 bladder cancer cells on the invasion, migration and proliferation of T24 cells
摘要
Abstract
Objective:To assess the effects of co-culturing human umbilical vein endothelial cells (HUVEC)with T24 bladder cancer cells on the invasion,migration and proliferation of T24 cells.Methods:We divided the study into two groups:a T24 bladder cancer cell group (T24 group) and the HUVEC-T24 bladder cancer cells trans-well co-culture group (co-culture group).The cell invasion,migration ability,and proliferation were compared using trans-well cell invasion Matrigel assay,transwell migration assay and CCK-8 proliferation assay.Results:After 24 hours,the number of invasive and migrated cells in the co-culture group were respectively 93.22±27.35 and 183.62±62.07,which were significantly higher than those in the T24 group (52.04±22.54 and 66.80±23.36) (P ＜0.01).The CCK-8 assay showed that the OD values on day 3,4 and 5were respectively (1.128±0.125),(0.866±0.068) and (1.536±0.098) in the co-culture group,and were (1.103±0.153),(1.910±0.139) and (1.550±0.177) in the T24 group.The cell proliferation rate was markedly higher in the co-culture group than the T24 group (P ＜0.01).Conclusion:Co-culture of HUVEC with T24 bladder cancer cells promotes the invasion,migration and proliferation of T24 cells.关键词
三维共培养模型/T24膀胱癌细胞/人脐静脉内皮细胞/侵袭/迁移/增殖Key words
co-culture model/T24 bladder cancer cells/human umbilical vein endothelial cells/invasion/migration/proliferation分类
医药卫生引用本文复制引用
高超,王琪,刘翰楠,吴健勇,李宇虹,庞波,梁霞,唐勇..三维共培养模型下人脐静脉内皮细胞对T24膀胱癌细胞侵袭、迁移及增殖能力的影响[J].广西医科大学学报,2017,34(3):337-341,5.基金项目
国家自然科学基金资助项目(No.81560428,No.81360341) （No.81560428,No.81360341）
广西自然科学基金资助项目(No.2011GXNSFC018020,No.2012GXNSFAA053163) （No.2011GXNSFC018020,No.2012GXNSFAA053163）
广西科学研究与技术开发计划项目(No.桂科攻1355005-3-11) （No.桂科攻1355005-3-11）
广西医疗卫生适宜技术研究与开发项目(No.S201301-06) （No.S201301-06）
广西卫生厅科研基金资助项目(No.Z2011226) （No.Z2011226）
