广西医科大学学报2017，Vol.34Issue(3)：350-354,5.DOI:10.16190/j.cnki.45-1211/r.2017.03.007
枸杞多糖联合替莫唑胺对大鼠脑胶质瘤的作用及机制研究
The effects and mechanisms of combined lyceum bararum polysaccharides with temozolomide on brain glioma in rats
李晓飞 1刘劲睿 1廉晓宇 1王德超 1张明石1
作者信息
- 1. 佳木斯大学附属第一医院神经外科,绥棱154001
- 折叠
摘要
Abstract
Objective:To explore the effects and mechanisms of combined lyceum bararum polysaccharides (LBP) with temozolomide (TMZ) on brain glioma in rats.Methods:SD rats were divided into 4 groups:control group,model group,TMZ group and LBP+TMZ group.The rats were intracranially inoculated with C6 glioma cells except for the controls.Rats in the treatment groups were administered TMZ or LBP plus TMZ via intragastric gavage,while those in the control and model groups were administered equal volume of normal saline.After 28 days administration,the rats were sacrificed and the tumor size was tested.The serum IL-17 level and Foxp3 mRNA expression were determined by enzyme-linked immuno sorbent assay (ELISA) and RT-PCR,respectively.The percentages of Th17 and Treg cells in glioma tissue were examined by flow cytometry.C6 glioma cells were subsequently cultured in vitro and divided into 3 groups:model group,TMZ group and LBP+TMZ group.After 72 h of cultivation,the IL-17 level in cell supernate and Foxp3 mRNA expression were also detected.Results:The size of tumors in the LBP+TMZ group were notably smaller than those in the TMZ group (P ＜0.05).The IL-17 level in rat serum and C6 glioma cells supernate were lower in the LBP+TMZ group than those in the TMZ group (P ＜0.05).The Foxp3 mRNA expression was down-regulated in the LBP+TMZ group than those in the model group and the TMZ group (P ＜0.05).The percentage of Treg cells was obviously reduced in the LBP+TMZ group compared to the TMZ group (P ＜0.05),and the ratio of Th17/Treg was significantly lower than those in the model group and the TMZ group (P ＜0.05).Conclusion:A combination administration of LBP and TMZ could effectively suppress the growth of brain glioma in rats via modulating Th17 and Treg cells.关键词
脑胶质瘤/枸杞多糖/替莫唑胺/T细胞Key words
brain glioma/lyciumbararum polysaccharides/temozolomide/T cells分类
医药卫生引用本文复制引用
李晓飞,刘劲睿,廉晓宇,王德超,张明石..枸杞多糖联合替莫唑胺对大鼠脑胶质瘤的作用及机制研究[J].广西医科大学学报,2017,34(3):350-354,5.
