广西植物2017，Vol.37Issue(2)：234-241,8.DOI:10.11931/guihaia.gxzw201601038
黄花蒿与其近缘种化学成分的FTIR和GC-MS鉴定与分析
GC-MS and FTIR identification and analysis of chemical component in Artemisia annua and its closely related species
摘要
Abstract
Artemisia annua contains abundant essential oils. It is commonly used as traditional Chinese medicine for trea-ting dysentery. The varietal complexity and vague provenance boundaries of the species of A. annua seriously affected the quality of medicinal materials. To investigate the rapid identification and evaluation of A. annua and sibling species lea ves on the yields and compositions of essential oils, the volatile compounds were detected and identified by GC-MS and FTIR analysis. A. annua determined to have the highest yield (1.86%) of essential oil, followed by A. eriopoda, A. cap-illarie, A. apiacea, A. japonica and A. argyi. FTIR results showed that one-dimensional spectrum was similarity of A. an-nua and sibling species and the species were abundant of amides, aromatics and terpenoids. Second derivative spectrum of A. annua component vibration absorption enhanced obviously to clearly demarcation of A. annua and confusion. GC-MS analysis showed that the volatile oil of A. annua and sibling species revealed the identification of 28 components and 17 common peaks. A. annua and sibling species leaf oils contained high amounts of camphor, á-Cadinene, crocetane, phytan, 2, 4-di-t-Butylphenol. The results indicated the apparent difference in the volatile compound compositions of es-sential oils between species. However, the content of phytane of A. annua was significantly higher than that of other spe-cies. Borneol was only detected in A. annua. In addition, A. apiacea, A. eriopoda and A. capillaries contained the highest á-Cedrene content, while A. annua, A. argyi and A. japonica contained the lowest content of á-cedrene. The oil compo-nents were analyzed using a hierarchical cluster for the six samples and samples were divided into two main clusters, A. annua, A. japonica and A. argyi was distinguished as a cluster, while A. apiacea, A. eriopoda and A. capillaries were classified as a cluster. These results provide an effective analysis methods for identifying the species, especially for the quality assessment for use in raw herbal medicines.关键词
黄花蒿/挥发油/GC-MS/红外图谱/质量评价Key words
Artemisia annua/essential oils/GC-MS/FTIR/quality assessment分类
农业科技引用本文复制引用
孔德鑫,李雁群,邹蓉,史艳财,韦霄..黄花蒿与其近缘种化学成分的FTIR和GC-MS鉴定与分析[J].广西植物,2017,37(2):234-241,8.基金项目
国家自然科学基金(81260623) （81260623）
广西科技成果转化与推广计划项目(桂科转 1346004-29) （桂科转 1346004-29）
广西植物所基本业务费项目(桂植业14017) [ Supported by the National Natural Science Foundation of China(81260623), Guangxi Scientific Research and Technological Development Plan (1346004-29) （桂植业14017）
Fundamental Research Foundation of Guangxi Insititute of Botany(14017)] . （14017）
