广西植物2017，Vol.37Issue(2)：242-247,241,7.DOI:10.11931/guihaia.gxzw201601039
地衣型真菌次级代谢产物抗菌活性初步研究
Antimicrobial activity of secondary metabolites from lichen forming fungi
摘要
Abstract
Lichen is a traditional ethnic medicine,which produces numerious active components. Thus, lichens have high research value. Most of antimicrobial ingredients from lichens were produced by lichen forming fungi. In order to detect the antimicrobial activity of secondary metabolites from lichen forming fungi, the fungi ( Xanthoria elegans, Myelochroa indica, Ramalina peruviana, Cladonia macilenta, Nephromopsis pallescens and Cladonia coccifera) were cultured in liq-uid medium for two months. The secondary metabolites of lichen forming fungi were obtained by extracting with ethyl ace-tate, and then the extracts were weighed and dissolved in DMSO. Seven kinds of pathogenic bacteria( Bacillus subtilis, B. cereus, Vibrio parahaemolyticus, Straphylococcus haemolyticus, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus) were used to screen activity of the extracts. Inoculating inhibition zone was used to evaluate the anti-bacterial activity of the extracts, and the minimum inhibitory concentration with antibacterial activity was also detected. The results showed that all of primary extract of lichen forming fungi had extensive antibacterial activity, and the antibacterial activity of the same fungi cultured showed significantly difference in different media. The secondary me-tabolites produced by Ramalina peruviana cultured in MY liquid medium had inhibitory effect to Straphylococcus aureus, Straphylococcus haemolyticus, Pseudomonas aeruginosa and Micrococcus luteus. It showed that the primary extract from Ramalina peruviana had extensive antibacterial effect. But the secondary metabolites of R. peruviana cultured in YMG liquid medium had not inhibitory effect to all of these pathogenic bacteria. Extracts from all of lichen forming fungi cul-tured in YMG liquid medium, Only the extract of Cladonia coccifera inhibited the Staphylococcus aureus which is gram-negative bacteria. The primary extract produced by Xanthoria elegans cultured in YMG medium had antibacterial effect to Bacillus cereus, the diameter of inhibition zone was up to 17.77 mm. Our results confirmed that the secondary metabolites of lichen forming fungi have antimicrobial activity and the lichen forming fungi can produce bioactive secondary metabo-lites in laboratorial culture conditions. Experiment results also showed that different media can stimulate different lichen forming fungi to produce different components. The innovation of this study was that different media were used to culture lichen forming fungi and lichen forming fungi had the characteristics of one strain many compounds. This study provides the groundwork to identify the active ingredients from lichens and provides an important information for culturing mass li-chens to obtain bioactive compounds.关键词
地衣型真菌/次级代谢产物/抗菌活性/抑菌圈法/液体培养Key words
Lichen forming fungi/secondary metabolites/antimicrobial activity/inoculating inhibition zone/liquid culture分类
生物科学引用本文复制引用
赵能,原晓龙,华梅,李苏雨,王娟,王毅..地衣型真菌次级代谢产物抗菌活性初步研究[J].广西植物,2017,37(2):242-247,241,7.基金项目
国家自然科学青年科学基金(31400488) （31400488）
云南省应用基础研究计划面上项目[Supported by the National Natural Science Foundation of China(31400488) （31400488）
the Science and Technology Planning Program of Yunnan Province]. （）
