工业微生物2017，Vol.47Issue(1)：10-16,7.DOI:10.3969/j.issn.1001-6678.2017.01.002
两阶段氮源补料策略促进微拟球藻EPA高效积累
Efficient accumulation of EPA from N.oculata by two stage feeding of nitrogen
摘要
Abstract
To improve the relative EPA content of fatty acid products from N.oculata,the effects of light intensity and the types of nitrogen source on the growth,biochemical composition and the relative contents of EPA were studied.Based on the experimental results of light intensity and nitrogen,the two stages of nitrogen source feeding experiments were designed and the timing of nitrogen feeding was optimized.The results showed that the specific growth rate increased from 0.25 d-1 to 0.54 d-1,the oil content increased from 11.4％ to 20.2％ while the EPA content decreased from 25.5％ to 13.1％ when the light intensity increased from 1 000 lux to 9 000 lux;ammonium acetate was found to be the best for the growth and oil of N.oculata,and its biomass could reach 0.41 g · L-1 and oil content was 14.3％,the EPA content of the nitrate group was highest,which reached 27.1％;By optimizing the timing of feeding of ammonium acetate,the biomass and relative EPA content of N.oculata reached 0.68 g · L-1 and 24.3 ％ respectively,which were higher than those of the control group by 15.3％ and 7.5％,and the optimum feeding time of ammonium acetate was the llth day.关键词
微拟球藻/EPA/光照强度/氮源种类/补料培养Key words
Nannochloropsis oculata C170/EPA/light intensity/types of nitrogen source/feeding culture引用本文复制引用
王玉仙,王鑫,赵犇,杨海麟..两阶段氮源补料策略促进微拟球藻EPA高效积累[J].工业微生物,2017,47(1):10-16,7.基金项目
国家863计划(2014AA021702)子课题 （2014AA021702）
江苏省2014年度普通高校研究生科研创新计划项目(KYLX_1148). （KYLX_1148）
