工业微生物2017，Vol.47Issue(1)：24-30,7.DOI:10.3969/j.issn.1001-6678.2017.01.004
γ-环糊精糖基转移酶在毕赤酵母中高效表达
High-level expression of codon optimized γ-cyclodextrin glycosyltransferase by Pichia pastoris
摘要
Abstract
In this work,the gene sequence of γ-cyclodextrin glycosyltransferase from Bacillus pseudalcaliphilus was optimized,the expression of Pichia pastoris was selected and its cultivation parameters were optimized for high level expression by Pichia pastoris.Under the conditions of pH 6.0,28 ℃,6.0％ inoculum size and 1.0％ methanol addition per 24 h,γ-cyclodextrin glycosyltransferase yield reached 83.65 U/mL,which was 8.7 folds of previous results.Finally,68.28％ γ-cyclodextrin glycosyltransferase was recovered through ammonium sulfate precipitation关键词
γ-环糊精糖基转移酶/密码子优化/毕赤酵母/发酵优化Key words
γ-cyclodextrin glycosyltransferase/codon optimization/Pichia pastoris/culture optimization引用本文复制引用
张燕,李梦腊,张建国..γ-环糊精糖基转移酶在毕赤酵母中高效表达[J].工业微生物,2017,47(1):24-30,7.基金项目
国家自然科学基金(No.21306112) （No.21306112）
上海市自然科学基金资助项目(No.13ZR1429100) （No.13ZR1429100）
上海高校青年教师培养资助计划(No.slg14037) （No.slg14037）
教育部留学回国人员科研启动基金. （）
