工业微生物2017，Vol.47Issue(1)：31-36,6.DOI:10.3969/j.issn.1001-6678.2017.01.005
谷氨酸棒杆菌S9114摇瓶发酵产γ-氨基丁酸的研究
Research of gamma aminobutyric acid production by Corynebacterium glutamicum S9114 in shaking flask
摘要
Abstract
In order to produce gamma-aminobutyric acid (GABA) by Corynebacterium glutamicum,metabolic engineering of GABA in reconmbinant C.glutamicum S9114 was investigated.By expressing exogenous gad genes (gadB1 and gadB2) from Lactobacillus brevis Lb85 which encoded glutamic acid decarboxylase (GAD),S9114/pJYW-4-gadBl-gadB2 was constructed.After fermented for 72 h,GABA production was 32.8 g/L and the conversion rate of glucose to GABA was 46.3％.By deleting alaT which encoded alanine aminotransferase,S9114△alaT/pJYW-4-gadB1-gadB2 was constructed.Fermentation results showed that the L-alanine production in S9114△alaT/pJYW-4-gadB1-gadB2 was 5.5％ lower than that in S9114/pJYW-4-gadB1-gadB2,which further reduced the content of the by-products.This study provided a valuable basis for the industrialized production of GABA by C.glutamicum.关键词
GABA/谷氨酸棒杆菌/S9114/alaTKey words
GABA/Corynebacterium glutamicum/S9114/alaT引用本文复制引用
斯晗,史锋..谷氨酸棒杆菌S9114摇瓶发酵产γ-氨基丁酸的研究[J].工业微生物,2017,47(1):31-36,6.基金项目
江南大学食品科学与技术国家重点实验室自由探索资助课题(编号SKLF-TS-201103). （编号SKLF-TS-201103）
