河北医学2017，Vol.23Issue(2)：228-232,5.DOI:10.3969/j.issn.1006-6233.2017.02.016
核酶阻断IGF-1及IGF-1R表达抑制病理性瘢痕形成的研究
Effect of Ribozyme on Blockade of IGF-1 and IGF-1R Expression as Well as Inhibition of Scar Formation
摘要
Abstract
Objective:To discuss insulin-like growth factor receptor 1 ( IGF-1) and its receptor ( IGF-1R) expression in scar tissue and the effect of ribozyme on the expression level. Methods:18 cases of Patho-logical scar tissue from operation excision (9 cases of scar keloid, 9 cases of scar hyperplasia ) were selected in Department of Burn and Plastics, Guangzhou Red Cross Hospital from 2015 May to 2016 July, meanwhile 20 cases of normal skin tissue specimens were collected. The expression level of tissue was observed by using hematoxylin eosin staining, the expression level of IGF-1 mRNA in each tissue specimen and its receptor mR-NA were detected by using real-time fluorescence quantitative PCR, scar tissue fibroblasts were derived to culture cells to obtained IGF-1 and its receptor ribozyme, the cells in the logarithmic growth phase with good growth state in 2-4 generation + ribozyme were selected to culture,72h later, the expression level of IGF-1 mRNA and its receptor mRNA were detected by using real-time fluorescence quantitative PCR. Results:The differences were statistically significant in the expression level of IGF-1 mRNA in normal skin, hypertrophic scar and keloid tissue and its receptor mRNA ( P <0.05) , the comparison between groups showed that normal skin hypertrophic scar tissue<hypertrophic scar tissue<scar keloid tissue and the differences were statistical-ly significant (P <0.05).Conclusion: The expression level of IGF-1 mRNA in scar tissue and its receptor mRNA are significantly higher than those in normal tissues, ribozyme has the effect on blockade of IGF-1 mR-NA and its receptor mRNA expression, which may play a role in inhibiting scar formation.关键词
胰岛素样生长因子受体1/瘢痕组织/核酶Key words
Insulin like growth factor receptor 1/Scar/Ribozyme引用本文复制引用
邓忠远,李孝建,侯海静,张涛..核酶阻断IGF-1及IGF-1R表达抑制病理性瘢痕形成的研究[J].河北医学,2017,23(2):228-232,5.基金项目
广东省医学科学技术研究基金项目,(编号:A2015069) （编号:A2015069）
