郑州大学学报（医学版）2017，Vol.52Issue(2)：109-112,4.DOI:10.13705/j.issn.1671-6825.2017.02.001
GRP78、CHOP在 α-细辛醚诱导的Eca109细胞凋亡中的作用
Effects of GRP78 and CHOP on apoptosis of human esophageal carcino-ma Eca109 cells induced by α-asarone
摘要
Abstract
Aim :To investigate the effect of GRP 78and CHOP on apoptosis of human esophageal carcinoma Eca 109 cell line induced by α-asarone.Methods:Eca109 cells were cultured for 24 h, and were treated with various concentra-tions(0.25, 0.50 and 1.00 g/L) ofα-asarone for 12, 24, 36 and 48 h, respectively.MTT assay was used to detect the proliferation of Eca109 cells.The apoptosis morphological changes of Eca 109 cells were observed with an inverted micro-scope.The expression level of GRP78 and CHOP were measured by Real-time PCR and Western blot, respectively.Re-sults:α-asarone had obvious time-and dose-dependent inhibition effects on the proliferation of Eca 109 cells(P<0.05). After Eca109 cells were treated with various concentrations of α-asarone for 48 h,the expressions of both GRP78 and CHOP were increased significantly(P<0.05).Conclusion:α-asarone could inhibit the proliferation of Eca109 cells and induce cell apoptosis by regulating the expressions of GRP 78 and CHOP.关键词
α-细辛醚/Eca109细胞/增殖抑制/凋亡/GRP78/CHOPKey words
α-asarone/Eca109 cell line/proliferation inhibition/apoptosis/GRP78/CHOP分类
医药卫生引用本文复制引用
王白燕,韩倩倩,朱艳琴,李光,石佳佳..GRP78、CHOP在 α-细辛醚诱导的Eca109细胞凋亡中的作用[J].郑州大学学报（医学版）,2017,52(2):109-112,4.基金项目
郑州市科技攻关项目 131PPTGG417-1 （）
郑州市科技创新团队项目 121PCXTD520 （）
