郑州大学学报（医学版）2017，Vol.52Issue(2)：129-134,6.DOI:10.13705/j.issn.1671-6825.2017.02.006
重症肌无力患者肌细胞中LRP4胞内区与SNX17的相互作用
Expression of LRP4 intracellular region and its interaction with SNX17 in muscle cells from patients with myasthenia gravis
摘要
Abstract
Aim: To explore the interaction between LRP 4 and SNX17 at the neuromuscular junction ( NMJ) in pa-tients with myasthenia gravis(MG).Methods:We constructed prokaryotic expression vector pET30a-LRP4 intracellular re-gion,transformed it into E.coli BL21( DE3) bacteria and induced expression of His-LRP4 intracellular region with relative molecular weight of about 17800;we constructed eukaryotic expression vector pEASY-Blunt M2-SNX17 , transfected it into HEK293 T cells and expressed Myc-SNX17 with relative molecular weight of about 53000 .Then we used two-way co-immu-noprecipitation to verify whether there was interaction between LRP 4 and SNX17.Results:We constructed prokaryotic ex-pression vector pET30a-LRP4 intracellular region and eukaryotic expression vector pEASY-Blunt M2-SNX17 successfully;Anti-Myc-tag mAb-magnetic beads mixed with His-LRP4 did not occur co-precipitation, while those mixed with Myc-SNX17 showed co-precipitation;Anti-His-tag mAb-magnetic beads mixed with Myc-SNX17 did not occur co-precipitation , while those mixed with His-LRP4 intracellular region showed co-precipitation.Conclusion: Prokaryotic expression LRP4 intra-cellular region and eukaryotic expression SNX 17 can combine directly in vitro.关键词
低密度脂蛋白受体相关蛋白4/分拣微管连接蛋白17/重症肌无力/免疫共沉淀Key words
low density lipoprotein receptor-related protein 4/sorting nexin 17/myasthenia gravis/co-immunoprecipi-tation分类
医药卫生引用本文复制引用
安颖,王金兰,张运克,吕杰,张迎娜,高峰,张婧,方华,李倩如,杜英,张清勇..重症肌无力患者肌细胞中LRP4胞内区与SNX17的相互作用[J].郑州大学学报（医学版）,2017,52(2):129-134,6.基金项目
国家自然科学基金资助项目 81471545 （）
河南省高等学校重点科研项目计划 16A320010,17A320046 （）
河南省科技攻关计划项目 1721023010298 （）
河南省高校科技创新团队项目 16IRTSTHN022 （）
